Prevalence of extended-spectrum beta-lactamase genes in Acinetobacter baumannii strains isolated from nosocomial infections in Tehran, Iran
Prevalence of extended-spectrum beta-lactamase genes in Acinetobacter baumannii strains isolated from nosocomial infections in Tehran, Iran
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DOI：
10.3205/dgkh000318
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发表时间：
2019-01-25
影响因子：
3
通讯作者：
Sedighi, Mansour
中科院分区：
文献类型：
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作者：
Abdar, Mojtaba Hanafi;Taheri-Kalani, Morovat;Sedighi, Mansour
背景和目标：bla（SHV）、bla（TEM）和bla（VEB）是一类能水解青霉素类和头孢菌素类抗生素的超广谱β-内酰胺酶（ESBLs）。本研究评估了ESBL基因bla（SHV）、bla（TEM）和bla（VEB）在鲍曼不动杆菌医院感染菌株中的频率，以概述这些基因在抗生素耐药中的重要性。采用Kirby-Bauer纸片扩散法进行耐药性评价后，采用E-test法测定环丙沙星的最低抑菌浓度（MIC）。采用纸片法对产ESBL菌株进行鉴定。结果：100株鲍曼不动杆菌中，表型法检测ESBL阳性率为59%。结论：产ESBLs的铜绿假单胞菌对大多数抗生素耐药率高，产ESBLs的铜绿假单胞菌中bla（TEM）基因的检出率高，而bla（SHV）和bla（VEB）基因的检出率低。目前研究中发现的鲍曼不动杆菌菌株引起了对伊朗医院感染的主要关注，因为这些菌株的治疗复杂性。我们的研究结果强调了感染控制措施的必要性，以防止耐药菌株的传播，特别是在医院。
Background and objectives: bla(SHV), bla(TEM) and bla(VEB) are a group of Extended-Spectrum Beta-Lactamase enzymes (ESBLs) which are able to hydrolyze Penicillins and some cephalosporin antibiotics. The present study evaluated the frequency of ESBL genes bla(SHV), bla(TEM) and bla(VEB) in Acinetobacter baumannii strains isolated from nosocomial infections to outline the importance of these genes in antibiotic resistance.Methods: One hundred Acinetobacter baumannii strains were isolated from different nosocomial infections. After antibiotic resistance evaluation with the Kirby-Bauer disc-diffusion method, the Minimum Inhibitory Concentration (MIC) of Ciprofloxacin was measured using the E-test method. Then, the ESBL producing strains were identified employing Combined Disk Methods. Finally, all isolates were evaluated with the Polymerase Chain Reaction (PCR) technique to detect the ESBL genes of interest.Results: Out of 100 Acinetobacter baumannii isolates, 59% were ESBL positive according to the phenotypic method. The PCR assay could not detect the bla(SHV) and bla(VEB) genes in the studied isolates, but the presence of bla(TEM) gene was demonstrated in 42% of the strains.Conclusion: The high resistance to most antibiotics, the high prevalence of ESBLs-producing strains and also a high prevalence of bla(TEM) gene in A. baumannii strains found in the current study gives cause for major concern about nosocomial infections in Iran because of the treatment complexity of these strains. Our results highlight the need for infection control measures to prevent the spread of resistant isolates, especially in hospitals.