Dynamics of Protein-ssDNA Interactions in the Bacteriophage T4 Homologous Recombination System
Dynamics of Protein-ssDNA Interactions in the Bacteriophage T4 Homologous Recombination System
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DOI：
10.1007/978-0-387-92808-1_10
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发表时间：
2011-01-01
期刊：
影响因子：
--
通讯作者：
Morrical, Scott W.
中科院分区：
文献类型：
--
作者：
Liu, Jie;Morrical, Scott W.
同源重组通过减数分裂和DNA双链断裂修复等过程在维持遗传多样性和基因组稳定性方面起着关键作用。同源重组的中心过程是DNA链交换，其中来自一条染色体的切除的断裂末端的单链DNA（ssDNA）侵入姐妹染色体的同源双链DNA（dsDNA）。这一反应是由突触前纤维催化的--核心重组蛋白的丝状复合物与入侵的ssDNA结合。成功的重组取决于这些蛋白质-ssDNA丝的协调组装和动力学。对噬菌体T4核心重组机制（UvsX重组酶、Gp 32 ssDNA结合蛋白、UvsY重组介体蛋白）的研究为同源重组中蛋白质-ssDNA相互作用的生物化学和生物物理学提供了有价值的见解。在这一章中，我们将探讨目前的模型的T4突触前纤维的组装和动态不稳定性，并显示该系统的机械功能可能会保存在其他重组系统。
Homologous recombination plays critical roles in maintaining genetic diversity and genome stability through processes such as meiosis and DNA double-strand break repair. The central process in homologous recombination is DNA strand exchange, in which single-stranded DNA (ssDNA) from the resected, broken end of one chromosome invades the homologous double-stranded DNA (dsDNA) of a sister chromosome. This reaction is catalyzed bypresynaptic filaments– filamentous complexes of core recombination proteins bound to the invading ssDNA. Successful recombination depends on the coordinated assembly and dynamics of these protein–ssDNA filaments. Studies of the bacteriophage T4 core recombination machinery (UvsX recombinase, Gp32 ssDNA-binding protein, UvsY recombination mediator protein) have provided valuable insights on the biochemistry and biophysics of protein–ssDNA interactions in homologous recombination. In this chapter, we explore current models for the assembly and dynamic instability of the T4 presynaptic filament and show how mechanistic features of this system may be conserved in other recombination systems.