Estimation of the human postmortem interval using an established rat mathematical model and multi-RNA markers
Estimation of the human postmortem interval using an established rat mathematical model and multi-RNA markers
复制标题
使用已建立的大鼠数学模型和多 RNA 标记来估计人类死后间隔
DOI：
10.1007/s12024-016-9827-4
复制
发表时间：
2017-03-01
影响因子：
1.8
通讯作者：
Chen, Long
中科院分区：
文献类型：
--
作者：
Lv, Ye-Hui;Ma, Jian-Long;Chen, Long
In our previous study, a R code-based mathematical model using RNA degradation patterns was developed for PMI determination in rat brain specimens. However, the postmortem changes of RNA are much more complicated in real cases, and there is still a huge challenge in efficiently applying information in animal data to real cases. In the present study, different RNA markers in both rat and human tissues were collected to screen valid biomarkers and the corresponding mathematical models were established and validated. With the same methodology, multi-RNA markers of myocardium and liver tissues were detected by qPCR and the Ct values of ten biomarkers generally increased with prolonged PMIs. 5S, miR-1 and miR-133a were shown to be optimum reference biomarkers that were not affected by a PMI of up to 5 or more days; however, liver-specific miR-122 began to degrade under higher temperatures and only 5S was selected as an endogenous control in the liver. Among the tested target RNAs, similar to our previous study in brain tissue, β-actin (ΔCt) was found to exhibit the best correlation coefficient with PMI and was employed to build mathematical models using R software. Following validation, the relatively low estimated error demonstrated that PMIs can be accurately predicted in human cases through comprehensive consideration of various factors and using effective biomarkers.
