Use of a parallel path nebulizer for capillary-based microseparation techniques coupled with an inductively coupled plasma mass spectrometer for speciation measurements
Use of a parallel path nebulizer for capillary-based microseparation techniques coupled with an inductively coupled plasma mass spectrometer for speciation measurements
复制标题
使用并行路径雾化器进行基于毛细管的微分离技术，并结合电感耦合等离子体质谱仪进行形态测量
DOI：
10.1016/j.sab.2004.03.005
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
N. J. Miller
中科院分区：
文献类型：
--
作者：
E. Yanes;N. J. Miller
设计用于毛细管电泳 (CE) 的低流量平行路径 Mira Mist CE 雾化器根据补充溶液流速、成分和浓度以及雾化器气体流速进行评估。这项研究是为了支持与使用微分离技术结合电感耦合等离子体质谱 (ICP-MS) 检测来分离和定量钴胺素（维生素 B-12）物质相关的项目而进行的。因此，在雾化器表征过程中监测 Co 信号。研究了 ICP 中的瞬态效应，以评估使用梯度进行微分离的适用性，并证明了使用甲醇作为补充溶液的好处。 Co 信号响应随着补充溶液中甲醇浓度的变化而显着变化，并且在 20% 甲醇、流速为 15 μl/min 时观察到最大信号增强。对改变雾化器气体流量效果的评估表明，氩气流量从 0.8 升/分钟到 1.2 升/分钟同样有效。然后评估 Mira Mist CE 平行路径雾化器与毛细管微分离技术的接口，包括毛细管电泳 (CE) 和微高效液相色谱 (μHPLC) 与电感耦合等离子体质谱 (ICP-MS)。使用 CE-ICP-MS 和 μHPLC-ICP-MS，使用平行路径雾化器和含有 20% 甲醇的补充溶液，流速为 15 μl/min，成功分离了四种钴胺素标准品（氰钴胺素、羟钴胺素、甲基钴胺素和 5' 脱氧腺苷钴胺素）和咕啉类似物钴胺二氰化物的混合物。
A low flow, parallel path Mira Mist CE nebulizer designed for capillary electrophoresis (CE) was evaluated as a function of make-up solution flow rate, composition, and concentration, as well as the nebulizer gas flow rate. This research was conducted in support of a project related to the separation and quantification of cobalamin (vitamin B-12) species using microseparation techniques combined with inductively coupled plasma mass spectrometry (ICP-MS) detection. As such, Co signals were monitored during the nebulizer characterization process. Transient effects in the ICP were studied to evaluate the suitability of using gradients for microseparations and the benefit of using methanol for the make-up solution was demonstrated. Co signal response changed significantly as a function of changing methanol concentrations of the make-up solution and maximum signal enhancement was seen at 20% methanol with a 15 μl/min flow rate. Evaluation of the effect of changing the nebulizer gas flow rates showed that argon flows from 0.8 to 1.2 l/min were equally effective. The Mira Mist CE parallel path nebulizer was then evaluated for interfacing capillary microseparation techniques including capillary electrophoresis (CE) and micro high performance liquid chromatography (μHPLC) to inductively coupled plasma mass spectrometry (ICP-MS). A mixture of four cobalamin species standards (cyanocobalamin, hydroxocobalamin, methylcobalamin, and 5′ deoxyadenosylcobalamin) and the corrinoid analogue cobinamide dicyanide were successfully separated using both CE-ICP-MS and μHPLC-ICP-MS using the parallel path nebulizer with a make-up solution containing 20% methanol with a flow rate of 15 μl/min.