Photogenerated lectin sensors produced by thiol-ene/yne photo-click chemistry in aqueous solution.
Photogenerated lectin sensors produced by thiol-ene/yne photo-click chemistry in aqueous solution.
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DOI：
10.1016/j.bios.2012.01.001
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发表时间：
2012-04-15
影响因子：
12.6
通讯作者：
Ramstrom, Olof
中科院分区：
文献类型：
--
作者：
Norberg, Oscar;Lee, Irene H.;Aastrup, Teodor;Yan, Mingdi;Ramstrom, Olof
硫醇和烯烃/炔之间的光引发的自由基反应（硫醇-烯和硫醇-炔化学）已被应用于功能化方法，以产生用于分析生物分子相互作用的碳水化合物呈递表面。聚合物涂覆的石英表面官能化的烯烃或炔在一个简单的光化学过程中，利用全氟苯基叠氮化物（PFPA）的化学。随后使烯烃/炔表面与碳水化合物硫醇在水中在UV照射下反应。该反应可以在没有光引发剂的情况下直接在表面上的一滴水中进行，并且任何二硫化物副产物在官能化过程之后容易地被洗掉。使用石英晶体微天平流通系统，在蛋白质-碳水化合物相互作用的实时研究中评价所得的碳水化合物呈递表面，所述系统具有使用低pH缓冲液的中间再生步骤的所选凝集素的重复注射。所得到的方法被证明是快速、有效和可扩展的高通量分析格式，并且所产生的表面显示出显著的蛋白质结合，具有研究中使用的凝集素的预期选择性。
The photoinitiated radical reactions between thiols and alkenes/alkynes (thiol-ene and thiol-yne chemistry) have been applied to a functionalization methodology to produce carbohydrate-presenting surfaces for analyses of biomolecular interactions. Polymer-coated quartz surfaces were functionalized with alkenes or alkynes in a straightforward photochemical procedure utilizing perfluorophenylazide (PFPA) chemistry. The alkene/alkyne surfaces were subsequently allowed to react with carbohydrate thiols in water under UV-irradiation. The reaction can be carried out in a drop of water directly on the surface without photoinitiator and any disulfide side products were easily washed away after the functionalization process. The resulting carbohydrate-presenting surfaces were evaluated in real-time studies of protein-carbohydrate interactions using a quartz crystal microbalance flow-through system with recurring injections of selected lectins with intermediate regeneration steps using low pH buffer. The resulting methodology proved fast, efficient and scalable to high-throughput analysis formats, and the produced surfaces showed significant protein binding with expected selectivities of the lectins used in the study.
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影响因子：
--
作者：
Davis, BG;Maughan, MAT;Jones, JB
通讯作者：
Jones, JB
DOI：
10.1016/s0021-9673(01)89570-4
发表时间：
1990-02-21
期刊：
JOURNAL OF CHROMATOGRAPHY
影响因子：
--
作者：
BHATTACHARYYA, L;BREWER, CF
通讯作者：
BREWER, CF
影响因子：
5.5
作者：
Fairbanks, Benjamin D.;Sims, Evan A.;Bowman, Christopher N.
通讯作者：
Bowman, Christopher N.
影响因子：
3.3
作者：
Hoorelbeke B;Van Damme EJ;Rougé P;Schols D;Van Laethem K;Fouquaert E;Balzarini J
通讯作者：
Balzarini J
影响因子：
4.8
作者：
Earl, Lesley A.;Bi, Shuguang;Baum, Linda G.
通讯作者：
Baum, Linda G.