5' Domain and nucleotide sequence of an adult chicken chromosomal beta-globin gene.
5' Domain and nucleotide sequence of an adult chicken chromosomal beta-globin gene.
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成年鸡染色体 β-珠蛋白基因的 5 结构域和核苷酸序列。
DOI：
10.1021/bi00511a005
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发表时间：
1981
期刊：
影响因子：
2.9
通讯作者：
S. Woo
中科院分区：
文献类型：
--
作者：
L. Day;A. J. Hirst;E. Lai;M. Mace;S. Woo
劳伦斯·E作者：Abigail J. Hirst，尤金C. Lai，Myles Mace，Jr.，和Savio L. C.从经EcoR Ⅰ酶切的鸡总DNA中克隆了一个含有成年3-珠蛋白基因的6.1-内切酶鸡染色体DNA片段。完整的3-珠蛋白基因长约1600个核苷酸，两侧分别为0.9和3。在该片段的5 ′和3 ′端分别有6个碱基对。鸡3-珠蛋白在与哺乳动物珠蛋白基因相同的位置含有两个插入序列。通过电镜和SI核酸酶图谱分析确定了珠蛋白基因的CAP位点，并建立了该基因5 '端的核苷酸序列。该基因在其5 '非翻译区含有80个核苷酸。在CAP位点之前的29个核苷酸处是Hogness盒序列GATAAAA。这个富含AT的区域周围的核苷酸序列非常富含GC（70%）。CG二核苷酸在这一区域的出现异常频繁，这导致了重组DNA技术允许对真核基因组进行解剖。通过分子克隆进行基因分离后的结构和测序分析已经确定哺乳动物珠蛋白基因由三个mRNA编码区段组成，这三个mRNA编码区段由两个间插序列隔开（Tilghman等人，1978; Tiemeier等人，1978; Leder等人，1978; Konkel等人，1979; Nishioka & Leder，1979; Lawn等人，1978; Lacy等人，1979; Hardison等人，1979;货车den贝格等人，1978年; Bernards等人，1979年）。最近，在成年鸡3-珠蛋白中也观察到这样的结构
Lawrence E. Day,* Abigail J. Hirst, Eugene C. Lai, Myles Mace, Jr., and Savio L. C. Woo* abstract: A 6.1-kilobase chromosomal chicken DNA fragment containing an adult 3-globin gene has been cloned from total EcoRI-digestedchicken DNA. The complete 3-globin gene is approximately 1600 nucleotides in lengthand is flanked by 0.9 and3. 6 kilobases of DNA inthis cloned fragment at its 5'and 3'termini, respectively. The chicken 3-globin contains two intervening sequences at the same locations as the mammalian globin genes. The CAP site of the globin gene was determined by electron microscopic and SI nuclease mapping, and the nucleotide sequence at the 5'end of the gene was established. The gene contains 80 nucleotides at its 5'-untranslated region. At 29 nucleotides preceding the CAP site is the Hogness box sequence GATAAAA. The nucleotide sequences surrounding this AT-rich region are extremely GC rich (70%). The occurrence of CG dinucleotides is unusually frequent in this region, resulting in the appearance, imme-^ Recombinant DNA technology has permitted the dissection of the eucaryotic genome. Structural and sequencing analyses after gene isolation by molecular cloninghave established that mammalian globin genes are comprised of three mRNA coding segments separated by two intervening sequences (Tilghman et al., 1978; Tiemeier et al., 1978; Leder et al., 1978; Konkel et al., 1979; Nishioka & Leder, 1979; Lawn et al., 1978; Lacy et al., 1979; Hardison et al., 1979; van den Berg et al., 1978; Bernards et al., 1979). More recently, such a structure has also been observed in an adult chicken 3-globin