Human CIK Cells Loaded with Au Nanorods as a Theranostic Platform for Targeted Photoacoustic Imaging and Enhanced Immunotherapy and Photothermal Therapy.
Human CIK Cells Loaded with Au Nanorods as a Theranostic Platform for Targeted Photoacoustic Imaging and Enhanced Immunotherapy and Photothermal Therapy.
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DOI：
10.1186/s11671-016-1468-8
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发表时间：
2016-12
影响因子：
--
通讯作者：
Cui D
中科院分区：
文献类型：
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作者：
Yang Y;Zhang J;Xia F;Zhang C;Qian Q;Zhi X;Yue C;Sun R;Cheng S;Fang S;Jin W;Yang Y;Cui D
如何实现胃癌的靶向光声成像、增强免疫治疗和光热治疗已成为一个巨大的挑战。本文首次报道了负载金纳米棒的人细胞因子诱导杀伤细胞（CIK）用于胃癌的靶向光声成像、增强免疫治疗和光热治疗。制备了二氧化硅修饰金纳米棒；然后与人细胞因子诱导的杀伤细胞（CIK）孵育，评价负载金纳米棒的人CIK细胞的细胞毒性、体外和体内胃癌靶向能力、免疫治疗和光热治疗效果。体外细胞实验表明，在低功率密度近红外（NIR）激光（808 nm连续波激光，1.5 W/cm2, 3 min）治疗下，金纳米棒标记的人CIK细胞积极靶向胃癌MGC803细胞，通过诱导细胞凋亡抑制MGC803细胞的生长，并杀死MGC803细胞。体内实验结果表明，金纳米棒标记的人CIK细胞在注射后4小时可通过光声成像主动靶向胃癌皮下血管，通过上调IL-1、IL-12、IL-2、IL-4、IL-17、IFN-γ等细胞因子，提高免疫治疗效果，并在近红外激光照射下直接注射到肿瘤部位，通过光热疗法杀死胃癌组织。金纳米棒标记的高性能人CIK细胞是一个很好的新型治疗平台，在不久的将来在肿瘤靶向光声成像、增强免疫治疗和光热治疗等方面具有很大的应用潜力。
How to realize targeted photoacoustic imaging, enhanced immunotherapy, and photothermal therapy of gastric cancer has become a great challenge. Herein, we reported for the first time that human cytokine-induced killer cells (CIK) loaded with gold nanorods were used for targeted photoacoustic imaging, enhanced immunotherapy, and photothermal therapy of gastric cancer. Silica-modified gold nanorods were prepared; then incubated with human cytokine-induced killer cells (CIK), resultant human CIK cells loaded with Au nanorods were evaluated for their cytotoxicity, targeted ability of gastric cancer in vitro and in vivo, immunotherapy, and photothermal therapy efficacy. In vitro cell experiment shows that human CIK cells labeled with gold nanorods actively target gastric cancer MGC803 cells, inhibit growth of MGC803 cells by inducing cell apoptosis, and kill MGC803 cells under low power density near-infrared (NIR) laser treatment (808-nm continuous wave laser, 1.5 W/cm2, 3 min). In vivo experiment results showed that human CIK cells labeled with gold nanorods could target actively and image subcutaneous gastric cancer vessels via photoacoustic imaging at 4 h post-injection, could enhance immunotherapy efficacy by up-regulating cytokines such as IL-1, IL-12, IL-2, IL-4, IL-17, and IFN-γ, and kill gastric cancer tissues by photothermal therapy via direct injection into tumor site under near-infrared (NIR) laser irradiation. High-performance human CIK cells labeled with Au nanorods are a good novel theranostic platform to exhibit great potential in applications such as tumor-targeted photoacoustic imaging, enhanced immunotherapy, and photothermal therapy in the near future.