Phylogenomic analysis of the PEBP gene family in cereals
Phylogenomic analysis of the PEBP gene family in cereals
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DOI：
10.1007/s00239-004-0179-4
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发表时间：
2005-11-01
影响因子：
3.9
通讯作者：
Damerval, C
中科院分区：
文献类型：
--
作者：
Chardon, F;Damerval, C
TFL 1和FT基因是控制拟南芥开花时间的关键基因，属于一个多基因小家族，其特征是具有特定的磷脂酰乙醇胺结合蛋白结构域，称为PEBP基因家族。在双子叶植物和单子叶植物中发现了几个PEBP基因，并控制开花时间。我们研究了PEBP基因家族在谷物中的进化。首先，利用水稻全基因组序列和EST数据库，我们在该物种中发现了19个PEBP基因，其中6个以前没有描述过。10个基因对应于5对旁系同源物映射在水稻基因组的已知重复区域。拟南芥和水稻基因的系统发育分析表明，PEBP基因家族由三个主要的同源类（所谓的TFL 1-LIKE，MFT-LIKE和FT-LIKE亚家族），其中基因重复和/或丢失独立发生在拟南芥和水稻。其次，对五种禾谷类植物基因组和EST序列的系统发育分析表明，PEBP基因的三个亚家族在禾谷类植物中是保守的。树状结构表明，祖先草基因组至少有两个MFT样基因，两个TFL 1样基因和八个FT样基因。一个新的基因组学方法导致了一些关于亚家族内基因功能保守的假设。
The TFL1 and FT genes, which are key genes in the control of flowering time in Arabidopsis thaliana, belong to a small multigene family characterized by a specific phosphatidylethanolamine-binding protein domain, termed the PEBP gene family. Several PEBP genes are found in dicots and monocots, and act on the control of flowering time. We investigated the evolution of the PEBP gene family in cereals. First, taking advantage of the complete rice genome sequence and EST databases, we found 19 PEBP genes in this species, 6 of which were not previously described. Ten genes correspond to five pairs of paralogs mapped on known duplicated regions of the rice genome. Phylogenetic analysis of Arabidopsis and rice genes indicates that the PEBP gene family consists of three main homology classes (the so-called TFL1-LIKE, MFT-LIKE, and FT-LIKE subfamilies), in which gene duplication and/or loss occurred independently in Arabidopsis and rice. Second, phylogenetic analyses of genomic and EST sequences from five cereal species indicate that the three subfamilies of PEBP genes have been conserved in cereals. The tree structure suggests that the ancestral grass genome had at least two MFT-like genes, two TFL1-like genes, and eight FT-like genes. A phylogenomic approach leads to some hypotheses about conservation of gene function within the subfamilies.