Composition and transport properties of human ankle and knee cartilage
Composition and transport properties of human ankle and knee cartilage
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DOI：
10.1002/jor.20029
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发表时间：
2006-02-01
影响因子：
2.8
通讯作者：
Nunley, JA
中科院分区：
文献类型：
--
作者：
Fetter, NL;Leddy, HA;Nunley, JA
与踝关节相比，膝关节的骨关节炎发病率明显更高，这表明这些关节软骨特性的差异可能导致骨关节炎的发生。作为一种无血管组织，关节软骨主要依赖于分子运输的扩散。本研究的目的是确定踝关节和膝关节软骨结构和组成的差异是否也反映为溶质运输特性的差异。采用光漂白后荧光恢复法（FRAP）测定70 kda葡聚糖分子在人踝关节和膝关节软骨中的扩散系数和分配系数，并与各区域内的蛋白聚糖、胶原蛋白、水和DNA含量进行比较。踝关节的平均分流系数显著低于膝关节（0.010 +/- 0.002 vs 0.022 +/- 0.003, p < 0.01），但扩散系数无显著差异(34.6 +/- 0.9 μ m(2)s(-1) vs 35.4 +/- 2.4 μ m（2)s(-1), p=0.70）。踝关节软骨的蛋白聚糖含量较高，且含水量有降低的趋势，说明踝关节软骨的有效孔径小于膝关节软骨。这些发现表明，踝关节和膝关节软骨组成的差异导致了分区系数的差异。本研究结果进一步支持了软骨的转运特性可能通过改变软骨细胞所暴露的生长因子和细胞因子的有效浓度，在这些关节骨关节炎发病率的差异中发挥作用的假设。(c) 2005年骨科研究学会。Wiley期刊公司出版。
The incidence of osteoarthritis is significantly higher in the knee as compared to the ankle, suggesting that differences in the properties of cartilage from these joints may contribute to the development of osteoarthritis. As an avascular tissue, articular cartilage depends primarily upon diffusion for molecular transport. The goal of this study was to determine if differences in the structure and composition between ankle and knee cartilage were also reflected as differences in solute transport properties. The diffusion coefficient and partition coefficient of a 70-kDa dextran molecule were measured in human ankle and knee articular cartilage using fluorescence recovery after photobleaching (FRAP) and were compared to the proteoglycan, collagen, water, and DNA contents within each zone. The mean partition coefficient was significantly lower in the ankle compared to the knee (0.010 +/- 0.002 vs. 0.022 +/- 0.003, p < 0.01), but no differences in the diffusion coefficients were observed (34.6 +/- 0.9 mu m(2)s(-1) vs. 35.4 +/- 2.4 mu m(2)s(-1), p=0.70). Ankle cartilage exhibited higher proteoglycan content as well as a trend toward lower water content, suggesting that ankle cartilage has a smaller effective pore size than knee cartilage. These findings suggest that differences in the composition of ankle and knee cartilage contribute to a difference in the partition coefficient. The results of this study provide further support for the hypothesis that the transport properties of cartilage may play a role in the differences in the incidence of osteoarthritis in these joints by altering the effective concentration of growth factors and cytokines to which chondrocytes are exposed. (c) 2005 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.