The Bordetella Bfe system:: Growth and transcriptional response to siderophores, catechols, and neuroendocrine catecholamines
The Bordetella Bfe system:: Growth and transcriptional response to siderophores, catechols, and neuroendocrine catecholamines
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DOI：
10.1128/jb.00495-06
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发表时间：
2006-08-01
影响因子：
3.2
通讯作者：
Armstrong, Sandra K.
中科院分区：
文献类型：
--
作者：
Anderson, Mark T.;Armstrong, Sandra K.
支气管败血波氏杆菌和百日咳波氏杆菌对肠杆菌素铁的利用需要BfeA外膜受体。在缺铁生长条件下，bfeA的转录由Welt调节因子通过需要铁载体肠杆菌素的机制激活。在这项研究中，肠杆菌素诱导的bfeA转录被证明是TonB独立的。为了确定其他铁载体或非铁载体儿茶酚是否可以被We系统利用，测试了各种化合物促进缺铁B生长的能力。支气管败血症并诱导bfeA转录。BfeA受体转运铁盐螯铁蛋白、棒状杆菌素和合成铁载体TRENCAM和MECAM。Salmochelin和MECAM诱导铁饥饿博德特氏菌细胞中的bfeA转录，但棒状杆菌素和TRENCAM的诱导作用最小。神经内分泌的儿茶酚胺类物质肾上腺素、去甲肾上腺素和多巴胺表现出显著的诱导bfeA转录的能力。去甲肾上腺素治疗B。支气管败血症导致BfeR依赖性bfeA转录，升高的BfeA受体产生和生长刺激。邻苯二酚、卡比多巴和异丙肾上腺素同样是bfeA转录的强诱导剂，而酪胺和3，4-二羟基扁桃酸表现出低诱导活性。结果表明，诱导物结构需要邻苯二酚基团的功能，并且诱导bfeA转录的能力不一定与刺激细菌生长的能力相关。由BfeA受体转运的儿茶酚铁载体的范围扩大，证明了博德特氏菌铁回收系统的潜在多功能性。儿茶酚胺类神经递质激活bfeA转录并促进生长的发现表明博德特氏菌细胞可以感知呼吸上皮上的神经内分泌儿茶酚胺并可能从中受益。
Ferric enterobactin utilization by Bordetella bronchiseptica and Bordetella pertussis requires the BfeA outer membrane receptor. Under iron-depleted growth conditions, transcription of bfeA is activated by the Welt regulator by a mechanism requiring the siderophore enterobactin. In this study, enterobactin-inducible bfeA transcription was shown to be TonB independent. To determine whether other siderophores or nonsiderophore catechols could be utilized by the We system, various compounds were tested for the abilities to promote the growth of iron-starved B. bronchiseptica and induce bfeA transcription. The BfeA receptor transported ferric salmochelin, corynebactin, and the synthetic siderophores TRENCAM and MECAM. Salmochelin and MECAM induced bfeA transcription in iron-starved Bordetella cells, but induction by corynebactin and TRENCAM was minimal. The neuroendocrine catecholamines epinephrine, norepinephrine, and dopamine exhibited a remarkable capacity to induce transcription of bfeA. Norepinephrine treatment of B. bronchiseptica resulted in BfeR-dependent bfeA transcription, elevated BfeA receptor production, and growth stimulation. Pyrocatechol, carbidopa, and isoproterenol were similarly strong inducers of bfeA transcription, whereas tyramine and 3,4-dihydroxymandelic acid demonstrated low inducing activity. The results indicate that the inducer structure requires a catechol group for function and that the ability to induce bfeA transcription does not necessarily correlate with the ability to stimulate bacterial growth. The expanded range of catechol siderophores transported by the BfeA receptor demonstrates the potential versatility of the Bordetella We iron retrieval system. The finding that catecholamine neurotransmitters activate bfeA transcription and promote growth suggests that Bordetella cells can perceive and may benefit from neuroendocrine catecholamines on the respiratory epithelium.