Oxidative homeostasis in oocyte competence for in vitro embryo development
Oxidative homeostasis in oocyte competence for in vitro embryo development
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DOI：
10.1111/asj.13256
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发表时间：
2019-08-30
影响因子：
2
通讯作者：
Michelette Damiao, Bruno Cesar
中科院分区：
文献类型：
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作者：
de Almeida Barros, Fabiana Dakkach;Adona, Paulo Roberto;Michelette Damiao, Bruno Cesar
本研究的目的是评估活性氧（ROS）和谷胱甘肽（GSH）水平的卵母细胞从不同直径的卵泡和它们的相关性在体外生产的胚胎（IVPE）。根据卵泡直径[2-8（一般）、4-8（大）和2 < 4 mm（小）]抽吸牛卵巢。评价卵母细胞的ROS、GSH、体外成熟和IVPE水平。在大组卵母细胞（85.6 +/- 7.2和140.0 +/- 9.6）中观察到较高水平的ROS和GSH（p < 0.05），其次是普通组（81.1 +/- 10.5和134.3 +/- 7.8）和小组（73.5 +/- 10.1和125.0 +/- 10.6）。然而，ROS/GSH的比例在普通组、大组和小组之间没有差异（p > 0.05）。成熟度较高（p < 0.05）大组（87.8 +/- 3.0%）高于小剂量组（72.2 +/- 5.8%），但两者相似（P > 0.05）与常规组比较（82.2 +/- 2.5%），而大组的IVPE（57.3 ± 3.0%）高于普通组（44.7 ± 4.4%）和小组（34.0 ± 4.0%）（p < 0.05）。我们报告说，卵母细胞从大卵泡更胜任IVPE，而较高水平的ROS和GSH似乎与卵母细胞的能力，只要保持氧化稳态。
The objective of this study was to evaluate the levels of reactive oxygen species (ROS) and glutathione (GSH) in oocytes from follicles of different diameters and their relevance in the in vitro production of embryos (IVPE). Bovine ovaries were aspirated according to the diameter of the follicle [2-8 (general), 4-8 (large), and 2 < 4 mm (small)]. The oocytes were evaluated for levels of ROS, GSH, in vitro maturation, and IVPE. Higher levels of ROS and GSH were observed (p < 0.05) in oocytes of the large group (85.6 +/- 7.2 and 140.0 +/- 9.6) followed by those in the general (81.1 +/- 10.5 and 134.3 +/- 7.8) and small (73.5 +/- 10.1 and 125.0 +/- 10.6) groups. However, the proportion of ROS/GSH did not differ (p > 0.05) between the general, large, and small groups. The maturation was higher (p < 0.05) in the large group (87.8 +/- 3.0%) than in the small group (72.2 +/- 5.8%), but both were similar (p > 0.05) to that in the general group (82.2 +/- 2.5%), whereas the IVPE of the large group (57.3 +/- 3.0%) was higher (p < 0.05) than those in the general (44.7 +/- 4.4%) and small (34.0 +/- 4.0%) groups. We report that oocytes from large follicles are more competent for IVPE, whereas higher levels of ROS and GSH appear to be correlated with oocyte competence, as long as oxidative homeostasis is retained.