Protein Stability Determination (PSD): A Tool for Proteomics Analysis
Protein Stability Determination (PSD): A Tool for Proteomics Analysis
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DOI：
10.2174/1574893613666180315121614
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发表时间：
2019-01-01
影响因子：
4
通讯作者：
Maiti, Smarajit
中科院分区：
文献类型：
--
作者：
Panja, Anindya Sundar;Nag, Akash;Maiti, Smarajit
背景资料：蛋白质稳定性测定（PSD）是一个基于序列的生物信息学工具，它是利用大量的FASTA格式的蛋白质序列数据集开发的。PSD可用于元蛋白质组学数据的分析，有助于预测和设计热酶和介酶，为学术和工业目的。PSD也可以用来分析蛋白质序列，并预测它是否会在嗜热或在mesophilicenvironment.Method和结果稳定：这是支持任何操作系统的工具是在Java中设计的，它提供了一个用户友好的图形界面。它是一个简单的程序，可以预测蛋白质的热稳定性性质，准确率>90%。PSD还可以预测组成氨基酸的性质，即酸性或碱性和极性或非极性等。结论：PSD是高度能够确定的假设或未知肽的蛋白质的热稳定性状态，以及从任何已建立的数据库的元蛋白质组学数据。PSD驱动分析的效用包括对蛋白质的功能分配的预测。PSD还有助于设计具有用于功能稳定性的氨基酸的灵活组合的肽。私营部门司可在https://sourceforge.net/projects/protein-sequence-determination免费获得。
Background: Protein Stability Determination (PSD) is a sequence-based bioinformatics tool which was developed by utilizing a large input of datasets of protein sequences in FASTA format. The PSD can be used to analyze the meta-proteomics data which will help to predict and design thermozyme and mesozyme for academic and industrial purposes. The PSD also can be utilized to analyze the protein sequence and to predict whether it will be stable in thermophilic or in the mesophilic environment.Method and Results: This tool which is supported by any operating system is designed in Java and it provides a user-friendly graphical interface. It is a simple programme and can predict the thermostability nature of proteins with >90% accuracy. The PSD can also predict the nature of constituent amino acids i.e. acidic or basic and polar or nonpolar etc.Conclusion: PSD is highly capable to determine the thermostability status of a protein of hypothetical or unknown peptides as well as meta-proteomics data from any established database. The utilities of the PSD driven analyses include predictions on the functional assignment to a protein. The PSD also helps in designing peptides having flexible combinations of amino acids for functional stability. PSD is freely available at https://sourceforge.net/projects/protein-sequence-determination.