Intra-articular delivery of sinomenium encapsulated by chitosan microspheres and photo-crosslinked GelMA hydrogel ameliorates osteoarthritis by effectively regulating autophagy
Intra-articular delivery of sinomenium encapsulated by chitosan microspheres and photo-crosslinked GelMA hydrogel ameliorates osteoarthritis by effectively regulating autophagy
复制标题
关节内递送壳聚糖微球和光交联 GelMA 水凝胶包裹的青藤，通过有效调节自噬改善骨关节炎
DOI：
10.1016/j.biomaterials.2015.12.006
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发表时间：
2016-03-01
期刊：
影响因子：
14
通讯作者：
Fan, Shunwu
中科院分区：
文献类型：
--
作者：
Chen, Pengfei;Xia, Chen;Fan, Shunwu
在人类和小鼠的病理软骨中已经观察到自噬调节剂的表达降低。本研究旨在研究通过暴露于壳聚糖微球（CM -SIN）和光合链接的明胶甲基丙烯酸甲酯（Geima）水凝胶（geima）水果凝胶和评估CM -siN的靶标的sinem -sins nisiN ose ars -sin forse forse ase ars -sin ase siN的研究，促进软骨细胞自噬的协同治疗效应。首先，我们制造并表征了凝胶水凝胶和壳聚糖微球。接下来，我们测量了SIN对软骨细胞和离体模型中11-13诱导的软骨基质降解的影响。罪调解了IL1-13至少通过激活自噬引起的病理变化。此外，我们通过手术诱导小鼠的骨关节炎，并在关节内注射CM -SIN和GELMA。手术后4和8周评估软骨基质降解和软骨细胞自噬。 CM-sin和Gelma的结合处理使手术诱导的OA的进展。罪至少通过体内诱导自噬来改善软骨基质降解。我们的结果表明，注射凝胶水凝胶和CM -sin的组合可能是治疗骨关节炎患者的有前途的策略。 （c）2015 Elsevier Ltd.保留所有权利。
Reduced expression of autophagy regulators has been observed in pathological cartilage in humans and mice. The present study aimed to investigate the synergistic therapeutic effect of promotion of chondrocyte autophagy via exposure to sinomenium (SIN) encapsulated by chitosan microspheres (CM -SIN) and photo-crosslinked gelatin methacrylate (GeIMA) hydrogel, with the goal of evaluating CM -SIN as a treatment for patients with osteoarthritis. First, we fabricated and characterized GelMA hydrogels and chitosan microspheres. Next, we measured the effect of SIN on cartilage matrix degradation induced by 11-13 in chondrocytes and an ex vivo model. SIN ameliorated the pathological changes induced by IL1-13 at least partially through activation of autophagy. Moreover, we surgically induced osteoarthritis in mice, which were injected intra-articularly with CM -SIN and GelMA. Cartilage matrix degradation and chondrocyte autophagy were evaluated 4 and 8 weeks after surgery. Treatment with the combination of CM-SIN and GelMA retarded the progression of surgically induced OA. SIN ameliorated cartilage matrix degradation at least partially by inducing autophagy in vivo. Our results demonstrate that injection of the combination of GelMA hydrogel and CM -SIN could be a promising strategy for treating patients with osteoarthritis. (C) 2015 Elsevier Ltd. All rights reserved.