Determination of the small amount of proteins interacting with TiO2 nanotubes by AFM-measurement
Determination of the small amount of proteins interacting with TiO2 nanotubes by AFM-measurement
复制标题
通过 AFM 测量测定与 TiO2 纳米管相互作用的少量蛋白质
DOI：
10.1016/j.biomaterials.2018.11.013
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发表时间：
2019-02-01
期刊：
影响因子：
14
通讯作者：
Lu, Xiaohua
中科院分区：
文献类型：
--
作者：
Dong, Yihui;Ji, Xiaoyan;Lu, Xiaohua
检测与固体膜电极表面有效相互作用的少量蛋白质仍然是一个挑战。为了解决这个问题，在这项工作中，提出了一种结合粘附力和 AFM 测量的分子相互作用的新方法。选择与 TiO2 纳米管阵列 (TNA) 有效相互作用的细胞色素 c (Cyt C) 作为探针。在TNA表面（C-TNA）上有效相互作用的Cyt C分子的量范围为5.5x10(-12)至7.0x10(-12) mol/cm(2) (68.2-86.8 ng/cm(2))，并且它们与文献中通过电化学方法获得的值相当，这证明了这种基于AFM的方法的准确性。通过进行表面增强拉曼散射（SERS）测量和估计增强因子（EF）进一步验证了所提出方法的可靠性。这种基于相互作用的AFM方法可用于在生物传感器、生物催化和药物输送等应用中准确获取固体膜电极表面上的少量吸附物质。
Detecting the small amounts of proteins interacting effectively with the solid film electrodes surface still remains a challenge. To address this, in this work, a new approach was proposed by the combination of the adhesion forces and the molecular interaction measured with AFM. Cytochrome c (Cyt C) interacting effectively with TiO2 nanotube arrays (TNAs) was chosen as a probe. The amounts of Cyt C molecules interacting effectively on TNAs surface (C-TNA) range from 5.5x10(-12) to 7.0x10(-12) mol/cm(2) (68.2-86.8 ng/cm(2)) and they are comparable with the values obtained by the electrochemistry method in the literature, in evidence of the accuracy of this AFM-based approach. The reliability of the proposed approach was further verified by conducting Surface Enhanced Raman Scattering (SERS) measurements and estimating the enhancement factor (EF). This interaction-based AFM approach can be used to accurately obtain the small amounts of adsorbed substances on the solid film electrodes surface in the applications such as biosensors, biocatalysis, and drug delivery, etc.