Overexpression of the long non-coding RNA MEG3 impairs in vitro glioma cell proliferation
Overexpression of the long non-coding RNA MEG3 impairs in vitro glioma cell proliferation
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DOI：
10.1002/jcb.24055
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发表时间：
2012-06-01
影响因子：
4
通讯作者：
Sun, Piyun
中科院分区：
文献类型：
--
作者：
Wang, Pengjun;Ren, Zhongqiao;Sun, Piyun
神经胶质瘤是成人中枢神经系统中最常见的原发性脑肿瘤。母系表达基因3（MEG 3）是位于14 q32的印记基因，其编码与肿瘤发生相关的非编码RNA（ncRNA）。然而，关于MEG 3是否以及如何调节胶质瘤的发展知之甚少。本研究采用实时荧光定量PCR方法检测MEG 3在胶质瘤组织中的表达，并通过CCK-8法、流式细胞术和RNA免疫沉淀法确定MEG 3的生物学功能和靶基因。我们首先证明，MEG 3的表达在胶质瘤组织与相邻的正常组织相比，显着降低。此外，MEG 3的异位表达抑制了U251和U87 MG人胶质瘤细胞系的细胞增殖并促进了细胞凋亡。我们进一步证实了MEG 3与p53相关，并且这种关联是p53激活所必需的。这些结果提示MEG 3在胶质瘤的分子病因学中具有重要作用，并提示MEG 3在胶质瘤治疗中具有潜在的应用价值。J.细胞。113：18681874，2012. (C)2012 Wiley Periodicals，Inc.
Gliomas are the most common type of primary brain tumor in the central nervous system of adults. Maternally Expressed Gene 3 (MEG3) is an imprinted gene located at 14q32 that encodes a non-coding RNA (ncRNA) associated with tumorigenesis. However, little is known about whether and how MEG3 regulates glioma development. In the present study we assayed the expression of MEG3 in glioma tissue samples by real-time polymerase chain reaction assay, and defined the biological functions and target genes by CCK-8 assay, flow cytometry, and RNA immunoprecipitation. We first demonstrated that MEG3 expression was markedly decreased in glioma tissues compared with adjacent normal tissues. Moreover, ectopic expression of MEG3 inhibited cell proliferation and promoted cell apoptosis in U251 and U87 MG human glioma cell lines. We further verified that MEG3 was associated with p53 and that this association was required for p53 activation. These data suggest an important role of MEG3 in the molecular etiology of glioma and implicate the potential application of MEG3 in glioma therapy. J. Cell. Biochem. 113: 18681874, 2012. (C) 2012 Wiley Periodicals, Inc.