Expression of endothelin-1 in gingival epithelial cells
Expression of endothelin-1 in gingival epithelial cells
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DOI：
10.1034/j.1600-0765.2003.00668.x
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发表时间：
2003-08-01
影响因子：
3.5
通讯作者：
Takehara, T
中科院分区：
文献类型：
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作者：
Yamamoto, E;Awano, S;Takehara, T
目的和背景：内皮素-1（ET-1）是一种由21个氨基酸组成的具有多功能调节作用的多肽。ET-1在炎症过程中表达于多种细胞。本研究的目的是检测感染牙周病原体后口腔上皮细胞中ET-1的表达，并研究ET-1在人类发炎和未发炎牙龈组织中的存在。材料和方法：用牙龈卟啉单胞菌感染KB细胞，采用逆转录-聚合酶链反应（RT-PCR）和酶联免疫吸附试验（ELISA）检测ET-1的表达水平。采用免疫组化法检测患者牙龈组织中ET-1的表达。结果：牙龈卟啉单胞菌感染可诱导KB细胞ET-1的表达。另一方面，在炎症牙龈组织的上皮细胞和血管内皮细胞中观察到ET-1的强免疫反应性。结论：牙周炎时牙龈上皮细胞中ET-1的表达水平可能增强。
Objectives and background: Endothelin-1 (ET-1) is a 21-amino acid peptide with multifunctional regulation. ET-1 expresses in various cells during inflammation. The present study aimed to examine the ET-1 expression in oral epithelial cells after infection with the periodontal pathogen and to investigate the presence of ET-1 in human inflamed and uninflamed gingival tissues.Materials and methods: The KB cells were infected with Porphyromonas gingivalis and the expression level of ET-1 was examined using reverse transcription-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA). The immunohistochemical analysis of ET-1 was performed in gingival tissues obtained from patients. In addition, the ET-1 mRNA expression in each tissue was also investigated by RT-PCR.Results: The expression of ET-1 in KB cells was strongly induced by the P. gingivalis infection. On the other hand, the strong immunoreactivity for ET-1 was observed in the epithelium and vascular endothelial cells of the inflamed gingival tissue. Furthermore, the level of ET-1 mRNA was greater in the inflamed tissues.Conclusion: These results suggested that the expression level of ET-1 in gingival epithelial cells might be enhanced during the periodontal inflammation.