Cellular glycosylation of amphiphilic saccharide primer in liquid/liquid interface culture system employing fluorous solvents
Cellular glycosylation of amphiphilic saccharide primer in liquid/liquid interface culture system employing fluorous solvents
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使用氟溶剂的液/液界面培养系统中两亲性糖引物的细胞糖基化
DOI：
10.1016/j.jfluchem.2016.06.011
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发表时间：
2016
影响因子：
1.9
通讯作者：
Kenichi Hatanaka
中科院分区：
文献类型：
--
作者：
Amane Sutoh;Maria Carmelita Z. Kasuya;Kenichi Hatanaka
由于氟化合物具有极高的携氧能力，液/液（培养基/氟溶剂）界面培养体系是其在生物技术领域的一个有吸引力的应用。然而，在这样的系统中培养的细胞的代谢是否可以正常工作仍有待阐明。为了确定这些细胞的糖脂代谢是否受到界面处这种低粘附条件的影响，我们使用三种氟溶剂在液/液界面系统中培养的小鼠黑色素瘤B16细胞评价了合成假糖脂的细胞糖基化。结果显示，当在界面处使用Fluorinert™ FC-72或全氟萘烷培养时，糖引物被细胞唾液酸化，但不使用十二碳六环庚醇。本工作的结果证实，在水/氟培养系统的界面处培养的细胞的糖脂代谢与正常的水培养系统一样正常地起作用，但取决于所使用的氟溶剂的种类。神经节苷脂的生物合成没有受到损害，在一个不寻常的环境中，如没有一个固体基板的细胞附着和存在的全氟溶剂。
As fluorous compounds have extremely high oxygen-carrying capacity, liquid/liquid (culture medium/fluorous solvent) interface culture system is one of their attractive applications in the field of biotechnology. However, it remains to be elucidated whether the metabolism of the cells cultured in such a system could work properly. To determine whether glycolipid metabolism of these cells are affected by such a low adhesive condition at the interface, we evaluated cellular glycosylation of a synthetic pseudo-glycolipid by mouse melanoma B16 cells cultured in liquid/liquid interface system using three kinds of fluorous solvents. The results showed that the saccharide primer was sialylated by the cells when cultured at the interface using Fluorinert™ FC-72 or perfluorodecalin, but not with dodecafluoroheptanol. The results of this work confirmed that glycolipid metabolism of cells cultured at the interface of aqueous/fluorous culture system functioned properly as well as normal aqueous culture system but depending on the kind of fluorous solvent used. Ganglioside biosynthesis was not compromised amidst an unusual environment such as the absence of a solid substrate for cell attachment and the presence of a perfluorinated solvent.