Cell recycle batch fermentation of high-solid lignocellulose using a recombinant cellulase-displaying yeast strain for high yield ethanol production in consolidated bioprocessing
Cell recycle batch fermentation of high-solid lignocellulose using a recombinant cellulase-displaying yeast strain for high yield ethanol production in consolidated bioprocessing
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DOI：
10.1016/j.biortech.2012.07.025
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发表时间：
2013-05-01
影响因子：
11.4
通讯作者：
Kondo, Akihiko
中科院分区：
文献类型：
--
作者：
Matano, Yuki;Hasunuma, Tomohisa;Kondo, Akihiko
本研究的目的是开发一种高固体木质纤维素原料的细胞循环分批发酵（CRBF）方案。两相分离包括通过低速离心和固液分离粗去除木质纤维素残留物，能够有效地收集具有降低的木质素和灰分的酿酒酵母细胞。200 g/L稻草经水热预处理后，连续5批发酵，平均酒精效价为34.5 g/L。此外，纤维素酶在重组酵母细胞表面上的展示将乙醇滴度提高到42.2g/L。在5个循环的发酵后，仅3.3g/L的糖保留在发酵培养基中，因为纤维素酶在细胞表面上显示水解的纤维素，而不是由商业纤维素酶或游离分泌的纤维素酶水解。重组菌在5轮重复分批发酵中，发酵能力保持良好，以起始生物量为基础的理论产量为86.3%。(C)2012爱思唯尔有限公司保留所有权利。
The aim of this study is to develop a scheme of cell recycle batch fermentation (CRBF) of high-solid lignocellulosic materials. Two-phase separation consisting of rough removal of lignocellulosic residues by low-speed centrifugation and solid-liquid separation enabled effective collection of Saccharomyces cerevisiae cells with decreased lignin and ash. Five consecutive batch fermentation of 200 g/L rice straw hydrothermally pretreated led to an average ethanol titer of 34.5 g/L. Moreover, the display of cellulases on the recombinant yeast cell surface increased ethanol titer to 42.2 g/L. After, five-cycle fermentation, only 3.3 g/L sugar was retained in the fermentation medium, because cellulase displayed on the cell surface hydrolyzed cellulose that was not hydrolyzed by commercial cellulases or free secreted cellulases. Fermentation ability of the recombinant strain was successfully kept during a five-cycle repeated batch fermentation with 86.3% of theoretical yield based on starting biomass. (C) 2012 Elsevier Ltd. All rights reserved.