Defense from the Group A Streptococcus by active and passive vaccination with the streptococcal hemoprotein receptor.
Defense from the Group A Streptococcus by active and passive vaccination with the streptococcal hemoprotein receptor.
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DOI：
10.1093/infdis/jir149
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发表时间：
2011-06
期刊：
影响因子：
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通讯作者：
Ya-Shu Huang;M. Fisher;Ziyad Nasrawi;Z. Eichenbaum
中科院分区：
文献类型：
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作者：
Ya-Shu Huang;M. Fisher;Ziyad Nasrawi;Z. Eichenbaum
背景A组链球菌（GAS）原发性感染和后遗症的全球负担是相当大的，尽管仍然缺少广泛覆盖超可变GAS的免疫计划。我们评估的链球菌血红素蛋白受体（SHR），保守的链球菌蛋白，作为一种疫苗候选人对GAS感染。方法用纯化的Shr免疫小鼠，或用表达Shr的乳酸乳球菌滴鼻免疫小鼠。测定血清和分泌物中产生的体液反应。我们评估了主动或被动接种Shr后小鼠对GAS感染的保护作用，并测试了Shr抗血清的杀菌活性。结果腹腔接种Shr后，小鼠血清中观察到强烈的Shr特异性免疫球蛋白（IG）G反应。鼻内免疫引起血清中强烈的IgG反应和分泌物中特异性的伊加反应。两种模型中的SHR免疫均增强了对全身性GAS攻击的保护。兔Shr抗血清具有调理作用，在感染前给予Shr抗血清的小鼠的存活率显著高于正常兔血清处理的小鼠。结论：Shr是一种有前途的候选疫苗，能够在被动和主动接种模型中引发杀菌抗体应答并赋予针对全身性GAS感染的免疫力。
BACKGROUND The worldwide burden of the Group A Streptococcus (GAS) primary infection and sequelae is considerable, although immunization programs with broad coverage of the hyper variable GAS are still missing. We evaluate the streptococcal hemoprotein receptor (Shr), a conserved streptococcal protein, as a vaccine candidate against GAS infection. METHODS Mice were immunized intraperitoneally with purified Shr or intranasally with Shr-expressing Lactococcus lactis. The resulting humoral response in serum and secretions was determined. We evaluated protection from GAS infection in mice after active or passive vaccination with Shr, and Shr antiserum was tested for bactericidal activity. RESULTS A robust Shr-specific immunoglobulin (Ig) G response was observed in mouse serum after intraperitoneal vaccination with Shr. Intranasal immunization elicited both a strong IgG reaction in the serum and a specific IgA reaction in secretions. Shr immunization in both models allowed enhanced protection from systemic GAS challenge. Rabbit Shr antiserum was opsonizing, and mice that were administrated with Shr antiserum prior to the infection demonstrated a significantly higher survival rate than did mice treated with normal rabbit serum. CONCLUSIONS Shr is a promising vaccine candidate that is capable of eliciting bactericidal antibody response and conferring immunity against systemic GAS infection in both passive and active vaccination models.