Neural retina development in vitro. Effects of tissue extracts on cell survival and neuritic development in purified neuronal cultures.
Neural retina development in vitro. Effects of tissue extracts on cell survival and neuritic development in purified neuronal cultures.
复制标题
神经视网膜的体外发育。
DOI：
10.1159/000112660
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发表时间：
1982
影响因子：
2.9
通讯作者：
Adler,R
中科院分区：
文献类型：
--
作者：
Hyndman,AG;Adler,R
从8天的鸡胚神经视网膜纯化的神经元群体可以获得的多聚鸟氨酸或高粘性胶原蛋白，与培养基中含有血清或无血清的'N1'补充。基于细胞极性，将神经元细胞的形态学分类为六个类别，所述细胞极性由神经突从每个细胞索马出现的位点的数量定义。不同的培养条件，因此可以定量比较使用作为参数，不仅总神经元的数量，而且每个细胞类别的总神经元群体的相对贡献。本文对鸡胚视叶、端脑、心脏和肾脏的水提液对培养的神经元的作用进行了定性和定量研究。除了上面提到的六种不同的形态学类别的过程轴承神经元，一种细胞类型出现在文化支持的视叶或端脑胶原蛋白，这可能永远不会被识别的其他条件下测试的媒体。这些“G”细胞占神经元总数的约1%，其特征在于具有非常长的神经突，比其他细胞类型的神经突长约20倍。胶原蛋白上G细胞的出现是这两种提取物对视叶和端脑唯一的影响。无论是神经元的总数和平均神经突起的发展是更高的血清或提取物含有文化比单独支持N1培养基的文化。此外，血清和提取物共享的能力，使多聚鸟氨酸基质有能力支持神经元的生存在无血清培养基中-可能是通过结合的材料的基质。
Purified neuronal populations from 8-day chick embryo neural retina can be obtained on either polyornithine or highly adhesive collagen, with media containing either serum or serum-free ‘N1’ supplement. A system was developed for the morphological categorization of neuronal cells into six categories, based on cell polarities as defined by the number of sites at which neurites are seen to emerge from each cell soma. Different culture conditions could thus be quantitatively compared using as parameters not only total neuronal numbers, but also the relative contribution of each cell category to the total neuronal population. A qualitative and quantitative study of the effects of aqueous extracts from chick embryo optic lobe, telencephalon, heart or kidney on these cultured neurons was carried out. In addition to the six distinct morphological categories of process-bearing neurons mentioned above, a cell type appeared in cultures supported by optic lobe- or telencephalon-containing media on collagen, which could never be recognized under the other conditions tested. These ''G'' cells represent about 1% of the total number of neurons, and are characterized by having a very long neurite, approximately 20-fold longer than the neurites of other cell types. The appearance of G cells on collagen was the only effect of optic lobe and telencephalon that was unique to these two extracts. Both the total number of neurons and the average neurite development were higher in serum- or extract-containing cultures than in cultures supported by N1medium alone. Moreover, sera and extracts shared the ability to render polyornithine substrata competent to support neuronal survival in serum-free medium - probably through the binding of materials to the substratum.