Visually induced initiation of Drosophila innate courtship-like following pursuit is mediated by central excitatory state
Visually induced initiation of Drosophila innate courtship-like following pursuit is mediated by central excitatory state
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DOI：
10.1038/ncomms7457
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发表时间：
2015-03-01
影响因子：
16.6
通讯作者：
Yamamoto, Daisuke
中科院分区：
文献类型：
--
作者：
Kohatsu, Soh;Yamamoto, Daisuke
雄性果蝇的求偶仪式代表了一种由雌性来源的感官刺激启动的先天行为。在这里，我们报告说，移动的光点可以诱导求爱后的追求拴系野生型雄蝇提供的苍蝇是由光遗传学刺激特定的dsx表达的神经元簇在侧前脑（LPR）。也就是说，刺激的pC1神经元簇启动单侧翼伸展和振动的两侧，而刺激的pC21集群启动对侧翼显示。此外，刺激pC21而不是pC1神经元诱导腹部弯曲和长鼻延伸。Ca2+成像的pC1集群显示周期性的Ca2+上升，每一个对应于一个回合的雄性苍蝇在求偶。相比之下，群体饲养的fru突变体雄性在没有光遗传学启动的情况下表现出光点诱导的求偶追求。Ca2+成像显示增强的反应LPR神经元的视觉刺激的突变体，这表明一个神经相关的光点诱导的求爱行为。
The courtship ritual of male Drosophila represents an innate behaviour that is initiated by female-derived sensory stimuli. Here we report that moving light spots can induce courtship-like following pursuit in tethered wild-type male flies provided the fly is primed by optogenetic stimulation of specific dsx-expressing neuronal clusters in the lateral protocerebrum (LPR). Namely, stimulation of the pC1 neuronal cluster initiates unilateral wing extension and vibration of both sides, whereas stimulation of the pC2l cluster initiates only contralateral wing displays. In addition, stimulation of pC2l but not pC1 neurons induced abdominal bending and proboscis extension. Ca2+ imaging of the pC1 cluster revealed periodic Ca2+ rises, each corresponding to a turn of the male fly during courtship. In contrast, group-reared fru mutant males exhibit light spot-induced courtship pursuit without optogenetic priming. Ca2+ imaging revealed enhanced responses of LPR neurons to visual stimuli in the mutants, suggesting a neural correlate of the light spot-induced courtship behaviour.