Cellulase Production by Thermomonospora curvata Isolated from Municipal Solid Waste Compost

Cellulase Production by Thermomonospora curvata Isolated from Municipal Solid Waste Compost
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从城市固体废物堆肥中分离出的热单孢菌生产纤维素酶

DOI:
10.1128/am.22.2.147-152.1971
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发表时间:
1971
期刊:
Applied Microbiology
影响因子:
--
通讯作者:
F. Stutzenberger
F. Stutzenberger
中科院分区:
--
文献类型:
--
作者:
F. Stutzenberger

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从城市垃圾堆肥样品中分离到一株分解纤维的嗜热放线菌,经鉴定为弯曲热单孢菌。确定了曲霉在含有无机盐、纤维素和酵母膏的培养基中55℃培养时产纤维素酶的最佳条件。曲霉所产纤维素酶的最适pH和最适温度(pH 6.0和65℃)与从粗堆肥样品中提取的纤维素酶的最适pH和最适温度相同。这些相似性,再加上堆肥样品中曲霉的普遍存在及其在堆肥温度下的生长能力,表明该放线菌可能被认为是城市垃圾堆肥过程中的主要纤维素分解者。
A cellulolytic, thermophilic actinomycete (previously isolated from municipal refuse compost samples) was identified as Thermomonospora curvata. A determination was made of the optimal conditions for cellulase production by T. curvata when grown at 55 C in a medium containing mineral salts, cellulose, and yeast extract. The pH and temperature optima (pH 6.0 and 65 C) for the cellulase produced by T. curvata were identical to those previously observed for the cellulase extracted from crude compost samples. Such similarities, together with the prevalence of T. curvata in compost samples and its ability to grow at composting temperatures, indicate that this actinomycete could possibly be considered as a major cellulose decomposer in the municipal refuse composting process.