THY-1-MEDIATED T-CELL ACTIVATION REQUIRES COEXPRESSION OF CD3/TI COMPLEX
THY-1-MEDIATED T-CELL ACTIVATION REQUIRES COEXPRESSION OF CD3/TI COMPLEX
复制标题
DOI：
10.1038/326505a0
复制
发表时间：
1987-04-02
期刊：
影响因子：
64.8
通讯作者：
SHEVACH, EM
中科院分区：
文献类型：
--
作者：
GUNTER, KC;GERMAIN, RN;SHEVACH, EM
除了抗CD3（T3）-T细胞抗原受体（CD3/Ti）复合物1 - 3的单克隆抗体外，其他几种针对人（CD2（T11）4和Tp 445，6）和鼠（Thy-17 - 9、TAP 10和Ly-611）T淋巴细胞上不同细胞表面结构的单克隆抗体能够激活T细胞。已经提出，这样的结构可以作为刺激的替代途径。为了直接检查Thy-1依赖性激活现象和通过CD 3/Ti复合物介导的T细胞激活之间是否存在任何关系，我们用鼠Thy-1.2基因转染了人T细胞系Jurkat的几种CD 3/Ti变体12，13。我们的数据表明，在CD3/Ti-，Thy-1.2+转染子中，抗Thy-1.2的单克隆抗体可以诱导胞浆游离钙（[Ca 2 +] i）升高，但不能刺激白细胞介素-2（IL-2）的产生。这些变异细胞系中导致不能响应Thy-1刺激产生IL-2的唯一缺陷是CD3/Ti复合物的表达，因为在IL-2产生测定中，缺陷Ti α-或β-链基因的替换重建了CD3/Ti的表面表达和对Thy-1的响应性。
In addition to monoclonal antibodies against the CD3 (T3)-T-cell antigen receptor (CD3/Ti) complex1–3, several other monoclonals directed towards distinct cell surface structures on human (CD2 (T11)4and Tp445,6) and murine (Thy-17–9, TAP10, and Ly-611) T lymphocytes are capable of activating T cells. It has been proposed that such structures may function as alternative pathways of stimulation. To examine directly whether any relationship exists between Thy-1-dependent activation phenomena and T-cell activation mediated through the CD3/Ti complex, we have transfected several CD3/Ti–variants12,13of the human T-cell line Jurkat with the murineThy-1.2gene. Our data indicate that in CD3/Ti–, Thy-1.2+transfectants, monoclonal antibodies against Thy-1.2 can induce a rise in cytoplasmic free calcium ([Ca2+]i), but fail to stimulate interleukin-2 (IL-2) production. The only defect in these variant cell lines responsible for the inability to produce IL-2 in response to Thy-1 stimulation was in the expression of the CD3/Ti complex, because replacement of defective Ti α- or β-chain genes reconstributed both surface expression of CD3/Ti and responsiveness to Thy-1 in the IL-2 production assay.