Combinational therapy with antibiotics and antibiotic-loaded adipose-derived stem cells reduce abscess formation in implant-related infection in rats
Combinational therapy with antibiotics and antibiotic-loaded adipose-derived stem cells reduce abscess formation in implant-related infection in rats
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DOI：
10.1038/s41598-020-68184-y
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发表时间：
2020-07-07
影响因子：
4.6
通讯作者：
Tsuchiya, Hiroyuki
中科院分区：
文献类型：
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作者：
Yoshitani, Junya;Kabata, Tamon;Tsuchiya, Hiroyuki
如果不延长抗生素疗程，植入物相关感染很难治疗。然而，抗生素的长期使用导致了耐多药和耐甲氧西林金黄色葡萄球菌的发展。因此，需要常规抗生素治疗的替代品。最近，间充质干细胞已被证明具有抗菌特性。本研究旨在评价大鼠种植体相关感染模型中，使用抗生素负载的脂肪干细胞（ADSC）加抗生素局部治疗的抗菌活性和治疗效果。液相色谱/串联质谱显示，ADSCs培养在环丙沙星的存在下，为24小时显示时间依赖性的抗生素负载。接下来，我们研究了ADSC和环丙沙星单独或组合在植入物相关感染大鼠模型中的治疗效果。将ADSC加抗生素、抗生素和ADSC的治疗效果与不治疗作为对照进行比较。在所有组中，ADSC+环丙沙星治疗的大鼠具有最低的改良骨髓炎评分、脓肿形成和植入物上的细菌负荷（P
Implant-related infection is difficult to treat without extended antibiotic courses. However, the long-term use of antibiotics has led to the development of multidrug- and methicillin-resistant Staphylococcusaureus. Thus, alternatives to conventional antibiotic therapy are needed. Recently, mesenchymal stem cells have been shown to have antimicrobial properties. This study aimed to evaluate the antimicrobial activity and therapeutic effect of local treatment with antibiotic-loaded adipose-derived stem cells (ADSCs) plus an antibiotic in a rat implant-associated infection model. Liquid chromatography/tandem mass spectrometry revealed that ADSCs cultured in the presence of ciprofloxacin for 24 h showed time-dependent antibiotic loading. Next, we studied the therapeutic effects of ADSCs and ciprofloxacin alone or in combination in an implant-related infection rat model. The therapeutic effects of ADSCs plus antibiotics, antibiotics, and ADSCs were compared with no treatment as a control. Rats treated with ADSCs plus ciprofloxacin had the lowest modified osteomyelitis scores, abscess formation, and bacterial burden on the implant among all groups (P