OXYTOCIN MEDIATES ATRIAL-NATRIURETIC-PEPTIDE RELEASE AND NATRIURESIS AFTER VOLUME EXPANSION IN THE RAT
OXYTOCIN MEDIATES ATRIAL-NATRIURETIC-PEPTIDE RELEASE AND NATRIURESIS AFTER VOLUME EXPANSION IN THE RAT
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DOI：
10.1073/pnas.92.17.7902
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发表时间：
1995-08-15
影响因子：
11.1
通讯作者：
ANTUNESRODRIGUES, J
中科院分区：
文献类型：
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作者：
HAANWINCKEL, MA;ELIAS, LK;ANTUNESRODRIGUES, J
我们以前的研究表明，前腹侧第三脑室区的刺激增加心房钠尿肽（ANP）的释放，而该结构的病变，正中隆起，或切除垂体的神经叶阻断由血容量扩张（BVE）引起的ANP释放。这些结果表明，中枢神经系统的参与是至关重要的，在这些反应，可能通过神经垂体激素的调解。在本研究中，我们调查了可能的作用，催产素，两个主要的神经垂体激素之一，在介导的心钠素释放。腹腔注射催产素（1-10 nmol）可引起尿渗透压、尿钠排泄和尿钾排泄的显著、剂量依赖性增加。还观察到延迟的抗利尿作用。腹腔注射缩宫素（10 nmol）后20 min，血浆心钠素浓度升高近4倍（P < 0.01），而对照组血浆心钠素浓度无明显变化。5 min时血浆ANP浓度也呈剂量依赖性升高（P < 0.001）。BVE心房内注射等渗盐水诱导血浆催产素和ANP浓度的快速（5分钟注射后）增加和血浆精氨酸加压素浓度的同时减少。结果与高渗性容量扩张相似，除了这引起血浆精氨酸加压素的短暂（5分钟）增加，研究结果与BVE刺激中枢神经系统压力感受器激活神经垂体释放催产素的假设一致。然后，催产素循环到右心房以诱导ANP的释放，ANP循环到肾脏并诱导钠尿和利尿，这将体液量恢复到正常水平。
Our previous studies have shown that stimulation of the anterior ventral third ventricular region increases atrial natriuretic peptide (ANP) release, whereas lesions of this structure, the median eminence, or removal of the neural lobe of the pituitary block ANP release induced by blood volume expansion (BVE). These results indicate that participation of the central nervous system is crucial in these responses, possibly through mediation by neurohypophysial hormones. In the present research we investigated the possible role of oxytocin, one of the two principal neurohypophysial hormones, in the mediation of ANP release. Oxytocin (1-10 nmol) injected i.p. caused significant, dose-dependent increases in urinary osmolality, natriuresis, and kaliuresis. A delayed antidiuretic effect was also observed. Plasma ANP concentrations increased nearly 4-fold (P < 0.01) 20 min after i.p. oxytocin (10 nmol), but there was no change in plasma ANP values in control rats, When oxytocin (1 or 10 nmol) was injected i.v., it also induced a dose-related increase in plasma ANP at 5 min (P < 0.001). BVE by intra-atrial injection of isotonic saline induced a rapid (5 min postinjection) increase in plasma oxytocin and ANP concentrations and a concomitant decrease in plasma arginine vasopressin concentration. Results were similar with hypertonic volume expansion, except that this induced a transient (5 min) increase in plasma arginine vasopressin, The findings are consistent with the hypothesis that baroreceptor activation of the central nervous system by BVE stimulates the release of oxytocin from the neurohypophysis. This oxytocin then circulates to the right atrium to induce release of ANP, which circulates to the kidney and induces natriuresis and diuresis, which restore body fluid volume to normal levels.