Developmental expression of the cucumber Cs-XTH1 and Cs-XTH3 genes, encoding xyloglucan endotransglucosylase/hydrolases, can be influenced by mechanical stimuli
Developmental expression of the cucumber Cs-XTH1 and Cs-XTH3 genes, encoding xyloglucan endotransglucosylase/hydrolases, can be influenced by mechanical stimuli
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编码木葡聚糖内转葡糖基酶/水解酶的黄瓜 Cs-XTH1 和 Cs-XTH3 基因的发育表达可能受到机械刺激的影响
DOI：
10.1007/s11738-018-2707-7
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发表时间：
2018
影响因子：
2.6
通讯作者：
Malinowski R
中科院分区：
文献类型：
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作者：
Malinowski R
在黄瓜体细胞胚胎发生过程中，编码木葡聚糖内切转葡糖基酶/水解酶（XTH）的两个基因Cs-XTH 1和Cs-XTH 3的表达上调。作为表征这些基因的发育调控的一种手段，在拟南芥和黄瓜的幼苗和体细胞胚中研究了各自上游调控区的活性。GUS检测表明，这两个基因都在发育控制之下。此外，在植物的张力承受区域以及对触摸和创伤的响应中发现启动子活性升高，这与在5′调控区存在大量与胁迫相关的顺式元件一致。进行体内木葡聚糖内切转葡糖基酶（XET）作用测定以获得XTH在体细胞胚胎发生期间的作用的概述。最高的XET行动中观察到的子叶区和PEG形成的假定区域的体细胞胚的外细胞层。基于这些结果，我们提出了一种双重机制（一种发育机制和第二种适应机制）来调节Cs-XTH 1和Cs-XTH 3的活性，其中发育模式可以通过机械刺激来改变。
The expression of two genes encoding xyloglucan endotransglucosylase/hydrolases (XTHs),Cs-XTH1andCs-XTH3, was upregulated during the onset of cucumber somatic embryogenesis. As a means of characterising the developmental regulation of these genes, the activity of the respective upstream regulatory regions was investigated in seedlings and somatic embryos ofArabidopsis thalianaandCucumis sativus. GUS assays revealed that both genes are under developmental control. In addition, elevated promoter activity was found in the tension-bearing regions of the plant and in response to touch and wounding, which is consistent with the existence of numerous stress-relatedciselements in the 5′-regulatory regions. In vivo xyloglucan endotransglucosylase (XET) action assays were performed to gain an overview on the role of XTHs during somatic embryogenesis. The highest XET action was observed in the external cell layers of somatic embryos in the cotyledonary region and in the presumptive region of peg formation. Based on the results, we propose a dual mechanism (one developmental and the second adaptive) for the regulation ofCs-XTH1andCs-XTH3activity wherein the developmental pattern can be modified by mechanical stimuli.