Identification and distribution of carbohydrate moieties on the salivary glands of Rhodnius prolixus and their possible involvement in attachment/invasion by Trypanosoma rangeli
Identification and distribution of carbohydrate moieties on the salivary glands of Rhodnius prolixus and their possible involvement in attachment/invasion by Trypanosoma rangeli
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DOI：
10.1016/s0014-4894(02)00026-7
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发表时间：
2002-04-01
影响因子：
2.1
通讯作者：
Ratcliffe, NA
中科院分区：
文献类型：
--
作者：
Basseri, HR;Tew, IF;Ratcliffe, NA
在本研究中，FITC标记的凝集素（WGA，Con A，PNA，HPA和TPA）被用来研究Rhodnius prolixus唾液腺表面的碳水化合物残基。结果表明，唾液腺是丰富的碳水化合物部分和特定的凝集素的结合模式的多样性表明，存在特定的碳水化合物残基在基膜，肌肉和细胞层的腺体。随后，利用唾液腺表面糖的检测结果，研究了兰氏锥虫与唾液腺表面糖的相互作用。唾液腺过多使用长外鞭毛体形式（侵袭/粘附形式）的体外粘附抑制试验表明，一些测试的糖能够阻断唾液腺表面和T. rangeli在测试的糖中，GlcNAc、GalNAc和半乳糖在唾液腺或寄生虫的预孵育后显示出最高的总体抑制作用。这些结果进行了讨论有关以前的工作中的碳水化合物和凝集素在昆虫载体/寄生虫相互作用的作用。
In the present study, FITC-labelled lectins (WGA, Con A, PNA, HPA, and TPA) were utilized to investigate carbohydrate residues on the surface of Rhodnius prolixus salivary glands. The results revealed that the salivary glands are rich in carbohydrate moieties and the diversity in binding pattern of particular lectins showed the presence of specific carbohydrate residues in the basal lamina, muscle, and cell layers of the glands. Subsequently, the sugars detected on the salivary gland surface were employed to investigate the interaction between Trypanosoma rangeli and the R. prolixus salivary glands. In vitro adhesion inhibition assays using long epimastigote forms (the invasion/adhesion forms) showed that some sugars tested were able to block the receptors on both the surfaces of the salivary glands and on T. rangeli. Among the sugars tested, GlcNAc, GalNAc, and galactose showed the highest overall inhibitory effect, following pre-incubation of either the salivary glands or parasites. These results are discussed in relation to previous work on the role of carbohydrates and lectins in insect vector/parasite interactions.