EVOLUTIONARY RELATIONSHIP BETWEEN THE NATURAL ANTI-GAL ANTIBODY AND THE GAL-ALPHA-1-]3GAL EPITOPE IN PRIMATES
EVOLUTIONARY RELATIONSHIP BETWEEN THE NATURAL ANTI-GAL ANTIBODY AND THE GAL-ALPHA-1-]3GAL EPITOPE IN PRIMATES
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DOI：
10.1073/pnas.84.5.1369
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发表时间：
1987-03-01
影响因子：
11.1
通讯作者：
MACHER, BA
中科院分区：
文献类型：
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作者：
GALILI, U;CLARK, MR;MACHER, BA
抗 Gal 是一种天然抗体，占人体循环 IgG 的 1%，并且对 Gal.alpha.1 .fwdarw 结构表现出明显的特异性。 3加仑。这种糖苷结构已在许多非灵长类哺乳动物的各种组织中发现。抗 Gal 的发生与 Gal.alpha.1 .fwdarw 表达的比较研究。在灵长类动物中进行了 3Gal 表位分析，观察到了独特的进化模式。尽管抗-Gal 被发现存在于旧世界猴和猿类 [Macaca mulatta、M. maura、M. fasciculis、M. nemestrina、M. arctoides、Papio papio 和 Cercopithecus diana] 中，滴度与人类相当，但其相应的抗原表位在新世界猴 [Callithrix] 的红细胞上大量表达。 jacchus、Aotus trivirgatus、Saguinus mystax、Saimiri sciureus、Cebus apella、Ateles paniscus、Alouatta caraya 和 Lagothrix lagothricha]。用来自新大陆猴红细胞的糖脂的抗 Gal 进行免疫染色表明，抗 Gal 结合的分子与兔和牛红细胞中发现的分子相似。这些发现表明，新世界和旧世界灵长类动物在 Gal.alpha.1 .fwdarw 的产生过程中存在进化互惠性。 3Gal 结构和识别它的抗体。 Gal.alpha.1 .fwdarw 的表达。 3Gal 表位在新世界猴中是进化保守的，但在旧世界灵长类动物的祖先谱系中受到抑制。该表位的抑制伴随着抗-Gal 的产生。观察到的抗-Gal 与人正常衰老红细胞和一些病理性红细胞的体内结合表明 Gal.alpha.1.fwdarw。 3Gal 表位以神秘形式存在于人体中。
Anti-Gal is a natural antibody, which constitutes as much as 1% of circulating IgG in humans and displays a distinct specificity for the structure Gal.alpha.1 .fwdarw. 3Gal. This glycosidic structure has been found on various tissues of many nonprimate mammals. A comparative study of the occurrence of anti-Gal versus the expression of the Gal.alpha.1 .fwdarw. 3Gal epitope was performed in primates, and a distinct evolutionary pattern was observed. Whereas anti-Gal was found to be present in Old World monkeys and apes [Macaca mulatta, M. maura, M. fascicularis, M. nemestrina, M. arctoides, Papio papio and Cercopithecus diana] in titers comparable to those in humans, its corresponding antigenic epitope is abundantly expressed on erythrocytes of New World monkeys [Callithrix jacchus, Aotus trivirgatus, Saguinus mystax, Saimiri sciureus, Cebus apella, Ateles paniscus, Alouatta caraya and Lagothrix lagothricha]. Immunostaining with anti-Gal of glycolipids from New World monkey erythrocytes indicated that the molecules to which anti-Gal binds are similar to those found in rabbit and bovine erythrocytes. These findings indicate that there is an evolutionary reciprocity between New World and Old World primates in the production of the Gal.alpha.1 .fwdarw. 3Gal structure and the antibody that recognizes it. The expression of the Gal.alpha.1 .fwdarw. 3Gal epitope was evolutonarily conserved in New World monkeys, but it was suppressed in ancestral lineages of Old World primates. The suppression of this epitope was accompanied by the production of anti-Gal. The observed in vivo binding of anti-Gal to human normal senescent and some pathologic erythrocytes implies that the Gal.alpha.1 .fwdarw. 3Gal epitope is present in man in a cryptic form.