RT-PCR Differentiation, Molecular and Pathological Characterization of Andean and Ordinary Strains of Potato virus S in Potatoes in China
RT-PCR Differentiation, Molecular and Pathological Characterization of Andean and Ordinary Strains of Potato virus S in Potatoes in China
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中国马铃薯马铃薯S病毒安第斯株和普通株的RT-PCR分化、分子和病理特征
DOI：
10.1094/pdis-11-15-1257-re
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发表时间：
2016-08-01
期刊：
影响因子：
4.5
通讯作者：
Nie, Bihua
中科院分区：
文献类型：
--
作者：
Wang, Jinghui;Meng, Fanye;Nie, Bihua
对湖北省马铃薯常见病毒的调查表明，马铃薯S病毒（PVS）是最丰富的病毒。为了揭示病毒的毒株身份，使用对PVS的普通和/或安第斯毒株特异的引物（即，PVSO和PVSA）。利用这些引物进行RT-PCR检测，成功地检测出了样品中的PVSO和PVSA。扩增子的序列分析证实了RT-PCR测定的正确性。选择分别代表PVSO和PVSA的两种分离株PVS HB 24和PVS HB 7进行分子和生物学表征。这两个分离株的基因组长度均为8,453 nt，有6个开放阅读框。它们在全基因组水平上的序列同一性为79.5%。系统发育分析将PVS HB 24和PVS HB 7分别置于PVSO和PVSA分支。PVS HB 24在接种藜的叶片上引起局部褪绿，而在未接种藜的上部叶片上无明显症状; PVS HB 7在接种藜的叶片上引起局部和全身症状。藜麦ELISA和RT-PCR证实PVS HB 7感染了C.而PVS HB 24不能这样做。这两个菌株感染马铃薯品种。Shepody和Solanum chacoense无症状地感染烟草，但不感染烟草和N. accn cv.萨姆森
A survey of potatoes in a field in Hubei, China, for common potato viruses revealed that Potato virus S (PVS) was the most abundant virus. To unveil the strain identity of the virus, primers specific to the ordinary and/or Andean strains of PVS (i.e., PVSO and PVSA) were designed. RT-PCR using these primers successfully detected PVSO and PVSA in the samples. Sequence analysis of the amplicons confirmed the correctness of the RT-PCR assay. Two isolates, PVS HB24 and PVS HB7, representing PVSO and PVSA, respectively, were chosen for molecular and biological characterization. Both isolates contained a genome of 8,453 nt in length with six open reading frames. They shared a sequence identity of 79.5% at the complete genome sequence level. Phylogenetic analysis placed PVS HB24 and PVS HB7 to PVSO and PVSA clades, respectively. PVS HB24 induced chlorotic local lesions on the inoculated leaves but no visible symptom on the upper uninoculated leaves of Chenopodium quinoa after mechanical inoculation, whereas PVS HB7 induced both local and systemic symptoms on C. quinoa. ELISA and RT-PCR confirmed that PVS HB7 infected C. quinoa systemically whereas PVS HB24 failed to do so. Both isolates infected potato cv. Shepody and Solanum chacoense asymptomatically, but did not infect Nicotiana occidentalis and N. tobaccwn cv. Samsun.