Application of viral-load kinetics to identify patients who develop cytomegalovirus disease after transplantation
Application of viral-load kinetics to identify patients who develop cytomegalovirus disease after transplantation
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DOI：
10.1016/s0140-6736(00)02350-3
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发表时间：
2000-06-10
期刊：
影响因子：
168.9
通讯作者：
Griffiths, PD
中科院分区：
文献类型：
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作者：
Emery, VC;Sabin, CA;Griffiths, PD
巨细胞病毒（CMV）仍然是移植后的主要问题；需要早期标志物来预测巨细胞病毒疾病的风险。血液中巨细胞病毒负荷峰值与巨细胞病毒疾病相关，但往往发生得太晚，无法提供预后信息。方法对359例移植受者（162例肝移植、87例肾移植、110例骨髓移植）采用定性和定量PCR方法进行前瞻性血液巨细胞病毒DNA检测。共分析了3873份样品。使用logistic回归评估第一个pcr阳性样本中的巨细胞病毒载量和复制初始阶段血液中巨细胞病毒载量的增长率作为巨细胞病毒疾病的危险因素。结果359例患者中127例血液中有巨细胞病毒DNA， 49例发展为巨细胞病毒疾病。初始病毒载量与CMV峰值载量显著相关(R-2=0.47, p=
Background Cytomegalovirus (CMV) continues to be a major problem post-transplantation; early markers for predicting patients at risk of CMV disease are needed. Peak CMV load in the blood correlates with CMV disease but frequently occurs too late to provide prognostic information.Methods 359 transplant recipients (162 liver, 87 renal, and 110 bone marrow) were prospectively monitored for CMV DNA in the blood with qualitative and quantitative PCR. 3873 samples were analysed. The CMV load in the first PCR-positive sample and the rate of increase in CMV load in blood during the initial phase of replication were assessed as risk factors for CMV disease using logistic regression.Findings 127 of the 359 patients had CMV DNA in the blood and 49 developed CMV disease. initial viral load correlated significantly with peak CMV load (R-2=0.47, p=