Dependency on light and vitamin A derivatives of the biogenesis of 3-hydroxyretinal and visual pigment in the compound eyes of Drosophila melanogaster.
Dependency on light and vitamin A derivatives of the biogenesis of 3-hydroxyretinal and visual pigment in the compound eyes of Drosophila melanogaster.
复制标题
果蝇复眼中 3-羟基视黄醛和视觉色素的生物发生对光和维生素 A 衍生物的依赖性。
DOI：
10.1085/jgp.92.5.587
复制
发表时间：
1988-11
影响因子：
3.8
通讯作者：
Tsukahara, Y
中科院分区：
文献类型：
--
作者：
Isono, K;Tanimura, T;Oda, Y;Tsukahara, Y
当果蝇，黑腹果蝇，培养基缺乏类胡萝卜素和类维生素A，3-羟基视网膜（果蝇视紫红质的发色团）在视网膜中的水平下降到低于1%，与正常苍蝇相比。3-hydroxyretinal的水平显着增加，在苍蝇的饮食中补充类维生素A或类胡萝卜素。喂食全反式维甲酸并保持在黑暗中的果蝇视网膜主要含有全反式形式的3-羟基视网膜，并且显示11-顺式异构体或视觉色素的水平没有增加。随后对果蝇的照射将大量的全反式3-羟基视网膜转化为其11-顺式异构体。通过照明的转换的作用光谱显示最佳波长为约420 nm，其显著大于游离的全反式3-羟基视网膜的最大吸收。在没有光的情况下，被喂食类胡萝卜素的苍蝇显示出11-顺式3-羟基视网膜和视觉色素水平的快速增加。
When the fruitfly, Drosophila melanogaster, was reared on media deficient in carotenoids and retinoids, the level of 3-hydroxyretinal (the chromophore of fly rhodopsin) in the retina decreased to less than 1% compared with normal flies. The level of 3-hydroxyretinal increased markedly in flies that were given a diet supplemented with retinoids or carotenoids. The retinas of flies fed on all-trans retinoids and maintained in the dark predominantly contained the all-trans form of 3- hydroxyretinal, and showed no increase in the level of either the 11- cis isomer or the visual pigment. Subsequent illumination of the flies converted substantial amounts of all-trans 3-hydroxyretinal to its 11- cis isomer. The action spectrum of the conversion by illumination showed the optimum wavelength to be approximately 420 nm, which is significantly greater than the absorption maximum of free, all-trans 3- hydroxyretinal. Flies that were fed on carotenoids showed a rapid increase of the levels of 11-cis 3-hydroxyretinal and of visual pigment in the absence of light.