3D material cytometry (3DMaC): a very high-replicate, high-throughput analytical method using microfabricated, shape-specific, cell-material niches.
3D material cytometry (3DMaC): a very high-replicate, high-throughput analytical method using microfabricated, shape-specific, cell-material niches.
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3D 材料细胞术 (3DMAC)：一种使用微加工、特定形状的细胞材料生态位的高重复性、高通量分析方法。
DOI：
10.1039/c7lc00451f
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发表时间：
2017
期刊：
影响因子：
6.1
通讯作者：
Roy,Krishnendu
中科院分区：
文献类型：
--
作者：
Parratt,Kirsten;Jeong,Jenny;Qiu,Peng;Roy,Krishnendu
研究3D材料壁龛内的细胞行为是理解细胞生物学在健康和疾病中的关键，并开发用于再生医学应用的生物材料。目前研究这些细胞材料生态位的方法吞吐量很低，每个实验只能分析几个重复，从而降低了测量保证和分析能力。在这里，我们报告了3D材料细胞术(3DMaC)，这是一种新的高通量方法，基于微制造的、特定形状的3D细胞材料壁龛和成像细胞术。3DMAC实现了对非常高的重复数(104-106的n)的3D生物材料构建物的快速和高度多元化的分析。3DMAC克服了目前低“n”、低通量和“噪音”分析的局限性，为3D生物材料培养中可能的数百个参数提供快速和同步的分析。该方法在这里演示了一组85 000事件，其中包含12个不同的细胞-生物材料微生态位，以及用于高通量分析的稳健、定制的计算方法，具有潜在的前所未有的统计能力。
Studying cell behavior within 3D material niches is key to understanding cell biology in health and diseases, and developing biomaterials for regenerative medicine applications. Current approaches to studying these cell–material niches have low throughput and can only analyze a few replicates per experiment resulting in reduced measurement assurance and analytical power. Here, we report 3D material cytometry (3DMaC), a novel high-throughput method based on microfabricated, shape-specific 3D cell–material niches and imaging cytometry. 3DMaC achieves rapid and highly multiplexed analyses of very high replicate numbers (“n” of 104–106) of 3D biomaterial constructs. 3DMaC overcomes current limitations of low “n”, low-throughput, and “noisy” assays, to provide rapid and simultaneous analyses of potentially hundreds of parameters in 3D biomaterial cultures. The method is demonstrated here for a set of 85 000 events containing twelve distinct cell–biomaterial micro-niches along with robust, customized computational methods for high-throughput analytics with potentially unprecedented statistical power.