Interordinal Chimera Formation Between Medaka and Zebrafish for Analyzing Stem Cell Differentiation
Interordinal Chimera Formation Between Medaka and Zebrafish for Analyzing Stem Cell Differentiation
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DOI：
10.1089/scd.2011.0630
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发表时间：
2012-08-01
影响因子：
4
通讯作者：
Hong, Yunhan
中科院分区：
文献类型：
--
作者：
Hong, Ni;Chen, Songlin;Hong, Yunhan
嵌合体形成是体内干细胞多能性的标准测试。远缘生物之间的种间嵌合体形成也为濒危物种的繁殖提供了一种有吸引力的方法。影响种间嵌合体形成的参数仍然很少被阐明。在这里，我们报道了青竹和斑马鱼之间的嵌合体形成，它们类似于3.2亿年前分离，并显示出发育速度的两倍以上的差异。我们发现，在移植到斑马鱼胚泡中，未培养的卵裂球和长期培养的青冈鱼胚胎干细胞都采用了斑马鱼的发育程序，并分化为具有生理功能的细胞类型，包括色素细胞、血细胞和心肌细胞。我们还发现，在嵌合胚胎发生过程中，青山ES细胞表达分化基因标志物。因此，不同的进化距离和不同的胚胎发生速度不会产生供体-宿主不亲和性，从而影响青海和斑马鱼嵌合体的形成，分子标记对于分析种间嵌合胚胎的谱系承诺和细胞分化是有价值的。
Chimera formation is a standard test for pluripotency of stem cells in vivo. Interspecific chimera formation between distantly related organisms offers also an attractive approach for propagating endangered species. Parameters influencing interspecies chimera formation have remained poorly elucidated. Here, we report interordinal chimera formation between medaka and zebrafish, which separated similar to 320 million years ago and exhibit a more than 2-fold difference in developmental speed. We show that, on transplantation into zebrafish blastulae, both noncultivated blastomeres and long-term cultivated embryonic stem (ES) cells of medaka adopted the zebrafish developmental program and differentiated into physiologically functional cell types including pigment cells, blood cells, and cardiomyocytes. We also show that medaka ES cells express differentiation gene markers during chimeric embryogenesis. Therefore, the evolutionary distance and different embryogenesis speeds do not produce donor-host incompatibility to compromise chimera formation between medaka and zebrafish, and molecular markers are valuable for analyzing lineage commitment and cell differentiation in interspecific chimeric embryos.