Microbiological evaluation of photo-activated disinfection in endodontics (An in vivo study)
Microbiological evaluation of photo-activated disinfection in endodontics (An in vivo study)
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DOI：
10.1038/sj.bdj.4813371
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发表时间：
2006-03-27
影响因子：
2.6
通讯作者：
Pearson, GJ
中科院分区：
文献类型：
--
作者：
Bonsor, SJ;Nichol, R;Pearson, GJ
目的观察光活化消毒（PAD）作为普通根管消毒的辅助手段在体内的微生物学效果。设计一项在普通牙科实践中进行的随机试验。研究对象和方法随机选择有不可逆性牙髓炎或根周牙周炎症状，需要进行根管治疗的患者。在常规根管治疗后，在进入根管时采集根管微生物样本，最后在制备的根管上进行PAD处理（光敏剂和光）后采集。每个管道的三个样本在取样30分钟内镀上，厌氧培养5天。记录每个样品的活菌生长情况，以确定细菌负荷。结果32条肛管中有30条纳入结果。其余两个的培养物没有在维持生存能力的目标时间内到达实验室。其余30条中，10条管培养阴性。这些要么是多根牙齿的一个管，其他的被感染，要么是在充血的重要组织上应用了多抗生素膏体的预处理。其余16例经常规牙髓治疗后培养阴性。4只感染的小鼠中有3只在PAD处理后培养为阴性。在一个仍存在可培养细菌的管道中，对光传输系统的审查表明，纤维的断裂使有效光输出减少了90%。结论在根管治疗中，使用PAD系统可有效杀灭常规冲洗液后残留的细菌。
Objective To determine the microbiological effect of photoactivated disinfection (PAD) as an adjunct to normal root canal disinfection in vivo.Design A randomised trial carried out in general dental practice.Subjects and methods Patients presenting with symptoms of irreversible pulpitis or periradicular periodontitis requiring endodontic therapy were selected at random. A microbiological sample of the canal was taken on accessing the canal, after conventional endodontic therapy, and finally after the PAD process (photosensitiser and light) had been carried out on the prepared canal. All three samples from each canal were plated within 30 minutes of sampling and cultured anaerobically for five days. Growth of viable bacteria was recorded for each sample to determine bacterial load.Results Thirty of the 32 canals were included in the results. Cultures from the remaining two did not reach the laboratory within the target time during which viability was sustained. Of the remaining 30, 10 canals were negative to culture. These were either one of the canals in multi rooted teeth where the others were infected or where a pre-treatment with a poly-antibiotic paste had been applied to hyperaemic vital tissue. Sixteen of the remainder were negative to culture after conventional endodontic therapy. Three of the four which had remained infected cultured negative after the PAD process. In the one canal where culturable bacteria were still present, a review of the light delivery system showed a fracture in the fibre reducing the effective light output by 90%.Conclusions The PAD system offers a means of destroying bacteria remaining after using conventional irrigants in endodontic therapy.