Quercetin inhibits epithelial-mesenchymal transition, decreases invasiveness and metastasis, and reverses IL-6 induced epithelial-mesenchymal transition, expression of MMP by inhibiting STAT3 signaling in pancreatic cancer cells.
Quercetin inhibits epithelial-mesenchymal transition, decreases invasiveness and metastasis, and reverses IL-6 induced epithelial-mesenchymal transition, expression of MMP by inhibiting STAT3 signaling in pancreatic cancer cells.
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槲皮素通过抑制胰腺癌细胞中的 STAT3 信号传导抑制上皮间质转化，降低侵袭性和转移性，并逆转 IL-6 诱导的上皮间质转化和 MMP 的表达
DOI：
10.2147/ott.s136840
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发表时间：
2017
影响因子：
4
通讯作者：
Zhou M
中科院分区：
文献类型：
--
作者：
Yu D;Ye T;Xiang Y;Shi Z;Zhang J;Lou B;Zhang F;Chen B;Zhou M
槲皮素是一种黄酮，具有多方面的作用，具有抗氧化、抗炎和抗癌的特性。本研究旨在探讨槲皮素对胰腺癌细胞上皮间质转化（EMT）和侵袭能力的影响及其机制。我们注意到槲皮素对PANC-1和帕图-8988细胞有明显的抑制作用。槲皮素还能抑制EMT和基质金属蛋白酶（MMP）的分泌。同时，我们测定了槲皮素处理后STAT 3的活性。槲皮素处理后STAT 3磷酸化降低。我们还使用STAT 3的活化剂IL-6诱导细胞恶性度的增加，并观察槲皮素的治疗效果。正如预期的那样，EMT和MMP分泌随着STAT 3信号通路的激活而增加，槲皮素逆转IL-6诱导的EMT、侵袭和迁移。因此，我们的研究结果表明，槲皮素触发抑制EMT，侵袭和转移通过阻断STAT 3信号通路，因此，槲皮素值得进一步研究。
Quercetin, a flavone, is multifaceted, having anti-oxidative, anti-inflammatory, and anticancer properties. In the present study, we explored the effects of quercetin on the epithelial–mesenchymal transition (EMT) and invasion of pancreatic cancer cells and the underlying mechanisms. We noted that quercetin exerted pronounced inhibitory effects in PANC-1 and PATU-8988 cells. Moreover, quercetin inhibited EMT and decreased the secretion of matrix metalloproteinase (MMP). Meanwhile, we determined the activity of STAT3 after quercetin treatment. STAT3 phosphorylation decreased following treatment with quercetin. We also used activating agent of STAT3, IL-6, to induce an increase in cell malignancy and to observe the effects of treatment with quercetin. As expected, the EMT and MMP secretion increased with activation of the STAT3 signaling pathway, and quercetin reversed IL-6-induced EMT, invasion, and migration. Therefore, our results demonstrate that quercetin triggers inhibition of EMT, invasion, and metastasis by blocking the STAT3 signaling pathway, and thus, quercetin merits further investigation.