Vascular corrosion casts mirroring early morphological changes that lead to the formation of saccular cerebral aneurysm: an experimental study in rats
Vascular corrosion casts mirroring early morphological changes that lead to the formation of saccular cerebral aneurysm: an experimental study in rats
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DOI：
10.3171/jns.2005.102.3.0532
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发表时间：
2005-03-01
影响因子：
4.1
通讯作者：
Satomi, J
中科院分区：
文献类型：
--
作者：
Jamous, MA;Nagahiro, S;Satomi, J
目的。脑动脉瘤的形成涉及复杂的过程，但对其起源、生长和破裂的机制知之甚少。本研究的目的是确定导致实验性脑动脉瘤形成的早期超微结构形态变化。方法。 20只雄性Sprague-Dawley大鼠进行脑动脉瘤诱导（肾性高血压和右颈总动脉结扎）； 10只完整大鼠作为对照组。 2个月后处死动物，制备脑动脉血管腐蚀铸件，并通过扫描电子显微镜筛查动脉瘤的发展。在脑动脉分叉处观察到响应血流动力学剪切应力的连续形态变化，包括内皮变化、内膜垫抬高和囊扩张。内皮细胞的变化是首先观察到的形态学变化；随后出现不同程度的动脉壁扩张。任何对照大鼠均未出现动脉瘤变化。在 20 只经过手术治疗的大鼠中，有 11 只表现出动脉瘤变化。在其中五只动物中，只能识别出内皮细胞印记的变化。在其他六只大鼠中，内皮细胞的形态变化与动脉瘤扩张的不同阶段相关。结论。这是第一项在体内证明导致脑动脉瘤形成的早期形态变化的研究。形态学研究结果表明内皮细胞在脑动脉瘤发病机制中的主要作用，并表明血流动力学剪切应力和血流模式可能会促成这些早期变化。
Object. The formation of cerebral aneurysms involves complex processes and little is known about the mechanisms by which they originate, grow, and rupture. The purpose of this study was to identify early ultrastructural morphological changes that lead to the formation of experimental cerebral aneurysms.Methods. Twenty male Sprague-Dawley rats were subjected to cerebral aneurysm induction (renal hypertension and right common carotid artery ligation); 10 intact rats served as the control group. The animals were killed after 2 months, and a vascular corrosion cast of their cerebral arteries was prepared and screened for aneurysm development by using a scanning electron microscope.Sequential morphological changes observed at the cerebral artery bifurcation in response to hemodynamic shear stress included endothelial changes, intimal pad elevation, and saccular dilation. Endothelial cell changes were the first observed morphological changes; they were followed by various degrees of artery wall dilation. No aneurysmal changes developed in any of the control rats. Of the 20 surgically treated rats, 11 displayed aneurysmal changes. In five of these animals only changes in the endothelial cell imprints could be identified. In the other six rats morphological changes in endothelial cells were associated with different stages of aneurysmal dilation.Conclusions. This is the first study to demonstrate in vivo early morphological changes that lead to the formation of cerebral aneurysms. The morphological findings indicate the principal role of endothelial cells in the pathogenesis of cerebral aneurysms and suggest that hemodynamic shear stress and blood flow patterns may precipitate these early changes.