Characterization of the protein subset desorbed by MALDI from whole bacterial cells
Characterization of the protein subset desorbed by MALDI from whole bacterial cells
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DOI：
10.1021/ac0008791
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发表时间：
2001-02-15
影响因子：
7.4
通讯作者：
Fenselau, C
中科院分区：
文献类型：
--
作者：
Ryzhov, V;Fenselau, C
这项研究描述了在分析整个细菌细胞时在MALDI质谱中检测到的蛋白质的各种特征，以了解为什么一些蛋白质被成功检测到，而许多其他蛋白质却没有。在大肠杆菌K-12和11775的光谱中，在4000-20 000 Da的质量范围内观察到的40个峰被暂时指定为蛋白质数据库中的蛋白质，并且这些蛋白质通过细胞位置、拷贝数、pi和疏水性来表征。所检测到的那些来源于胞质溶胶，并且通常具有细胞内高丰度、强碱性和中等亲水性的特征。
This study characterizes various features of the proteins that are detected in MALDI mass spectra when whole bacteria cells are analyzed, in an effort to understand why some proteins are successfully detected and many others are not. Forty peaks observed in the mass range 4000-20 000 Da in the spectra of Escherichia coli K-12 and 11775 are tentatively assigned to proteins in a protein database, and these proteins are characterized by cell location, copy number, pi, and hydropathicity. Those detected originate in the cytosol and generally share the traits of high abundance within the cell, strong bacisity, and medium hydrophilicity.