Quantitative analysis of small bio-samples of nearly 1 μg.
Quantitative analysis of small bio-samples of nearly 1 μg.
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对近 1 μg 的小生物样品进行定量分析。
DOI：
10.1142/s0129083514400099
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Matsumasa M
中科院分区：
文献类型：
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作者：
Sera K;Goto S;Takahashi C;Saitoh Y;Kinoshita K;Matsumasa M
我们开发和报告了各种生物样品在真空和空气中PIXE的无标准方法，它们已被应用于定量分析的痕量生物样品的重量小于0.1毫克。本研究建立了一种近真空PIXE定量分析生物样品的方法。为了在无标准方法的基础上提高对较小样品的分析的灵敏度和准确度，该方法利用从样品发射的连续X射线，必须设计用于支撑样品的适当背衬材料。在本研究中，我们已经检查了各种背衬材料，如较薄和线状背衬。结果发现，由几乎不含杂质的延长粘合剂制成的线状背衬最适合于极少量的生物样品，因为它不会产生大量的连续X射线。该方法已用于小型昆虫和植物的定量分析。此外，该方法还被应用于在海洋生态系统食物链中起重要作用的负鼠虾和虾蛄等小型海洋生物的分析。研究结果为遭受2011年3月11日大海啸袭击的三陆地区的海洋生态系统再生提供了有价值的信息。
We developed and reported standard-free methods for various bio-samples in both invacuum and in-air PIXE and they have been applied to quantitative analyses of traces of bio-samples whose weights are less than 0.1 mg. In this study, we established a method of quantitative analysis for bio-samples of nearlyin in-vacuum PIXE. In order to improve sensitivity and accuracy of analysis for smaller samples on the basis of the standard-free method, which makes use of continuous X-rays emitted from the sample, it is essential to design appropriate backing materials for supporting the sample. In the present study, we have examined various backing materials such as thinner and threadlike backings. As a result, it was found that a thread-like backing made of extended adhesive, which contains almost no impurity, is the most suitable for bio-samples of extremely small quantities, since it produces no large amount of continuous X-rays. The method has been applied to quantitative analyses of small insects and plants. Moreover, the method was applied to analyses of small marine organisms such as opossum shrimps and squilla shrimps which play important roles in a food chain in marine ecosystem. The results gave us valuable information about regeneration of marine ecosystem in the Sanriku district attacked by the huge tsunami that occurred on 11 March 2011.