ASSOCIATION OF EPIDERMAL LIGNIFICATION WITH NONHOST RESISTANCE OF CUCURBITS TO FUNGI
ASSOCIATION OF EPIDERMAL LIGNIFICATION WITH NONHOST RESISTANCE OF CUCURBITS TO FUNGI
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DOI：
10.1139/b85-342
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发表时间：
1985-12-01
期刊：
影响因子：
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通讯作者：
BAKER, KK
中科院分区：
文献类型：
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作者：
HAMMERSCHMIDT, R;BONNEN, AM;BAKER, KK
用炭疽菌接种黄瓜、甜瓜、西瓜、南瓜和南瓜的黄化苗。魔法。和Halst。第一种，炭疽菌（伯克）；和Br)。陶布。或Helminthosporium carbonum Ull。真菌在所有寄主上都能很好地萌发和产生附着胞。然而，只有在与黄瓜、甜瓜和西瓜的相互作用中，通常才观察到对宿主细胞的渗透。其他宿主-病原体相互作用（非宿主相互作用）的组织化学染色显示，真菌对非宿主组织的渗透不足与木质素沉积在附着胞周围的上表皮细胞壁和外侧表皮细胞壁有关。用0.5 M NaOH或热乙醇处理木质化组织不改变染色反应。用热氨基乙醇（一种去木质素剂）处理确实可以消除染色。铜氧化物对每个宿主木质化表皮细胞壁的氧化表明，每个宿主都产生了富含对香豆素、缺乏愈创木酰基的木质素样物质。这些结果表明木质素化可能是葫芦科植物普遍的非寄主抗性反应。
Etiolated seedlings of cucumber, muskmelon, watermelon, pumpkin, and squash were inoculated with Colletotrichum lagenarium (Pass.) Ell. and Halst. race 1, Colletotrichum atramentarium (Berk. and Br.) Taub., or Helminthosporium carbonum Ull. The fungi germinated and produced appressoria equally well on all hosts. Penetrations into host cells, however, were routinely observed only in the interactions of C. lagenarium with cucumber, muskmelon, and watermelon. Histochemical staining of the other host-pathogen interactions (nonhost interactions) revealed that the lack of penetration by fungi into nonhost tissue was associated with the deposition of lignin in the upper and lateral host epidermal cell walls around appressoria. Treatment of the lignified tissues with 0.5 M NaOH or hot ethanol did not alter staining reactions. Treatment with hot aminoethanol, a delignifying agent, did eliminate stainings. Cupric oxide oxidation of lignified epidermal cell walls from each host revealed that each host produced a p-coumaryl-rich, guaiacyl-poor ligninlike material. These results suggest that lignification may be a general nonhost resistance response in the Cucurbitaceae.