Transcription and Activity of Digestive Enzymes of Nezara viridula Maintained on Different Plant Diets
Transcription and Activity of Digestive Enzymes of Nezara viridula Maintained on Different Plant Diets
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DOI：
10.3389/fphys.2019.01553
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发表时间：
2020-01-08
影响因子：
4
通讯作者：
Bonning, Bryony C.
中科院分区：
文献类型：
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作者：
Canton, Pablo Emiliano;Bonning, Bryony C.
稻绿蝽（Nezara viridula）是一种以玉米、大豆和棉花等重要经济作物为食的杂食性蝽。为了增加对这种害虫在这种不同种植系统中取食的能力的了解，我们分析了玉米或绿色豆的独家饮食对消化酶的酶活性和转录组学谱的影响。与玉米相比，当昆虫只在绿色豆上饲养时，生长速率和存活率降低。然而，两种处理之间的总体蛋白酶和核酸酶活性谱相当。在某些情况下，抑制剂敏感性和活性存在明显差异，特别是对于中肠某些区域的丝氨酸蛋白酶。从N.在RNA-seq数据的主成分分析中，以玉米和绿色菜豆为食的viridula不同。虽然特定的转录差异转录根据饮食和跨几个组织进行了鉴定，大量的这些转录仍然未注释。这些基因的进一步注释将对进一步理解N. viridula
Nezara viridula is a polyphagous stink bug that feeds on crops of economic importance such as corn, soybean and cotton. To increase understanding of the ability of this pest insect to feed on such diverse cropping systems, we analyzed the impact of an exclusive diet of corn or green bean on the enzymatic activity and transcriptomic profile of digestive enzymes. Growth rate and survival were reduced when insects were reared exclusively on green bean compared to corn. However, the overall protease and nuclease activity profiles were comparable between the two treatments. Distinct differences in inhibitor sensitivity and activity were seen in some cases, particularly for serine proteases in some regions of the midgut. The transcription profiles from N. viridula fed on corn versus green bean were distinct on principal component analysis of RNA-seq data. While specific transcripts differentially transcribed according to diet and across several tissues were identified, a large number of these transcripts remain unannotated. Further annotation for identification of these genes will be important for improved understanding of the remarkable polyphagy of N. viridula.