Discrimination of Isomeric Carbohydrates as the Electron Transfer Products of Group II Cation Adducts by Ion Mobility Spectrometry and Tandem Mass Spectrometry
Discrimination of Isomeric Carbohydrates as the Electron Transfer Products of Group II Cation Adducts by Ion Mobility Spectrometry and Tandem Mass Spectrometry
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DOI：
10.1021/acs.analchem.5b00759
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发表时间：
2015-06-02
影响因子：
7.4
通讯作者：
Dodds, Eric D.
中科院分区：
文献类型：
--
作者：
Huang, Yuting;Dodds, Eric D.
异构碳水化合物结构的快速和明确的区别仍然是一个巨大的分析挑战。本文报道了第一次开发碳水化合物/金属离子相互作用与气相离子化学，以提高歧视的寡糖异构体的离子迁移谱和串联质谱。这是证明了两个异构的五糖和两个异构的六糖，每一个研究的未衍生化的形式作为其钙离子加合物，钡离子加合物，和气相电子转移产品。与适当选择的电荷载体，一个单一的电子转移到碳水化合物金属离子加合物导致异构体区分其离子/中性碰撞截面的位移和外观的独特功能，在其振动活化/解离光谱。这些研究结果表明，新的和优雅的气相策略，异构低聚糖的快速分化。
The rapid and unambiguous distinction of isomeric carbohydrate structures persists as a tremendous analytical challenge. This paper reports the first exploitation of carbohydrate/metal ion interactions in concert with gas-phase ion chemistry to improve discrimination of oligosaccharide isomers by both ion mobility spectrometry and tandem mass spectrometry. This is demonstrated for two isomeric pentasaccharides and two isomeric hexasaccharides, each studied in an underivatized form as their calcium ion adducts, barium ion adducts, and gas-phase electron transfer products thereof. With appropriate selection of the charge carrier, transfer of a single electron to the carbohydrate metal ion adducts resulted in isomer-distinguishing shifts in their ion/neutral collision cross sections and the appearance of unique features in their vibrational activation/dissociation spectra. These findings suggest novel and elegant gas-phase strategies for rapid differentiation of isomeric oligosaccharides.