Validation of a method for the targeted analysis of 96 drugs in hair by UPLC-MS/MS
Validation of a method for the targeted analysis of 96 drugs in hair by UPLC-MS/MS
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DOI：
10.1016/j.jpba.2013.08.050
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发表时间：
2014-01-25
影响因子：
3.4
通讯作者：
Nielsen, Marie Katrine Klose
中科院分区：
文献类型：
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作者：
Montesano, Camilla;Johansen, Sys Stybe;Nielsen, Marie Katrine Klose
在这项研究中提出的方法可以筛选和定量的96种药物，从不同的群体：阿片类药物，安非他明，致幻剂，苯二氮卓类药物，抗组胺药，抗抑郁药，抗精神病药，巴比妥类药物和其他镇静剂，肌肉松弛剂等。用甲醇：乙腈：甲酸铵的混合物（pH 5.3）在18小时内从10 mg洗过的头发中提取药物。75%分析物的绝对回收率范围为70%-106%。在低pg/mg范围内的检测限可允许检测单次给药药物暴露，可能应用于药物促进性攻击（DFA）;然而，也可检查长期使用（依从性）。该方法已被充分验证用于研究中包含的药物。该方法的准确性证明了认证的真实的头发样本含有常见的滥用药物的分析。毛发法具有广泛的潜力，因为目标分析物的测量范围宽，并且由于提取程序和色谱系统的多功能性，可以很容易地将新药添加到该方法中。(C)2013 Elsevier B. V.保留所有权利。
The method presented in this study allows the screening and quantification of 96 drugs, from different groups: opiates, amphetamines, hallucinogens, benzodiazepines, antihistamines, antidepressants, antipsychotics, barbiturates and other sedatives, muscle relaxants, etc. in hair. Drugs are extracted from 10 mg of washed hair in 18 h by a mixture of methanol:acetonitrile:ammonium formate (pH 5.3). Absolute recovery ranged from 70% to 106% for 75% of the analytes. The limits of detection in the low pg/mg range, may allow the detection of single dose drug exposure, with possible application in drug facilitated assaults (DFA); however, chronic use (compliance) can also be examined. The method has been fully validated for the drugs included in the study. The accuracy of the method was demonstrated by the analysis of certified authentic hair samples containing common drugs of abuse. The hair-method has broad potential as the measuring range is wide for the target analytes and new drugs can easily be added to the method due to the versatility of the extraction procedure and chromatographic system. (C) 2013 Elsevier B.V. All rights reserved.