Proanthocyanidins Extracted from Rhododendron pulchrum Leaves as Source of Tyrosinase Inhibitors: Structure, Activity, and Mechanism.
Proanthocyanidins Extracted from Rhododendron pulchrum Leaves as Source of Tyrosinase Inhibitors: Structure, Activity, and Mechanism.
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从杜鹃叶中提取的原花青素作为酪氨酸酶抑制剂的来源：结构、活性和机制
DOI：
10.1371/journal.pone.0145483
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Peng YY
中科院分区：
文献类型：
--
作者：
Chai WM;Wang R;Wei MK;Zou ZR;Deng RG;Liu WS;Peng YY
本研究旨在探讨从美丽杜鹃叶中提取的原花青素的结构、抗酪氨酸酶活性及其作用机制。基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）和高效液相色谱电喷雾质谱（HPLC-ESI-MS）的质谱分析结果表明，原花青素是原花青素、原飞燕草素、螺桨海葵素及其衍生物的复杂混合物，其中原花青素是主要成分。抗酪氨酸酶活性分析结果表明，该混合物为可逆的混合竞争性酪氨酸酶抑制剂。原花青素与底物（L-酪氨酸和3，4-二羟基苯丙氨酸）和铜离子之间的相互作用是解释其有效抑制的重要分子机制。本研究为R.作为新的酪氨酸酶抑制剂的美丽草叶原花色素。
The objective of this study was to assess the structure, anti-tyrosinase activity, and mechanism of proanthocyanidins extracted from Rhododendron pulchrum leaves. Results obtained from mass spectra of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and high performance liquid chromatography electrospray ionization mass spectrometry (HPLC-ESI-MS) revealed that proanthocyanidins were complex mixtures of procyanidins, prodelphinidins, propelargonidins, and their derivatives, among which procyanidins were the main components. The anti-tyrosinase analysis results indicated that the mixtures were reversible and mixed competitive inhibitors of tyrosinase. Interactions between proanthocyanidins with substrate (L-tyrosine and 3,4-dihydroxyphenylalanine) and with copper ions were the important molecular mechanisms for explaining their efficient inhibition. This research would provide scientific evidence for the use of R. pulchrum leaf proanthocyanidins as new novel tyrosinase inhibitors.