Identification of 28 DNA fragments that detect RFLPs in 13 distinct physical regions of the short arm of chromosome 5.
Identification of 28 DNA fragments that detect RFLPs in 13 distinct physical regions of the short arm of chromosome 5.
复制标题
鉴定 28 个 DNA 片段，用于检测 5 号染色体短臂 13 个不同物理区域中的 RFLP。
DOI：
10.1093/nar/15.11.4617
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发表时间：
1987
影响因子：
14.9
通讯作者：
Wasmuth,JJ
中科院分区：
文献类型：
--
作者：
Overhauser,J;McMahan,J;Wasmuth,JJ
从对人5号染色体短臂（5 p）特异的文库中分离的一系列175个携带人插入物的噬菌体，已经使用16个人-仓鼠细胞杂交体的缺失作图组在5 p上进行了区域作图，其中每一个都包含一个染色体B，该染色体在短臂中具有不同的缺失。用12种限制性内切酶筛选5个单拷贝DNA片段，以确定它们检测限制性片段长度多态性（RFLP）的能力。其中28个DNA片段位于5 p的13个不同的物理区域，被发现检测到RFLP。这些DNA标记使构建一个连锁图谱成为可能，该图谱将跨越整个5 p长度，并将允许以高分辨率检查基因组该区域的遗传距离和物理距离之间的关系。
A series of 175 lambdaphage carrying human inserts isolated from a library that is specific for the short arm of human chromosome 5 (5p) have been regionally mapped on 5p using a deletion mapping panel of 16 human-hamster cell hybrids, each of which contains a chromosome b with a different deletion in the short arm. Seventy-five single copy DNA fragments were screened with 12 restriction enzymes for their ability to detect restriction fragment length polymorphisms (RFLPs). Twenty-eight of these DNA fragments, which are located in 13 distinct physical regions of 5p, were found to detect RFLPs. These DNA markers make it possible to construct a linkage map that will span the entire length of 5p and will allow the relationship between genetic and physical distance for this region of the genome to be examined at a high level of resolution.