Aggregate formation and polysaccharide content of Chlorella pyrenoidosa Chick (Chlorophyta) in response to simulated nutrient stress.
Aggregate formation and polysaccharide content of Chlorella pyrenoidosa Chick (Chlorophyta) in response to simulated nutrient stress.
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DOI：
10.1016/j.biortech.2010.06.022
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发表时间：
2010-11
影响因子：
11.4
通讯作者：
Zhou Yang;Y. Liu;Juan Ge;Wei Wang;Yafen Chen;D. Montagnes
中科院分区：
文献类型：
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作者：
Zhou Yang;Y. Liu;Juan Ge;Wei Wang;Yafen Chen;D. Montagnes
为了确定多糖生产如何影响微藻聚集体的大小，我们使用现有的方法（以前应用于蓝藻和植物）刺激多糖生产。培养物用乙醛酸（0、0.25、0.5、1.25mmol/L）处理以模拟营养胁迫，并检查9d（在第9天接近稳定期）以评估：生长速率、多糖产生（可溶性、结合的和总的）、聚集体大小以及多糖和聚集体大小之间的关系。我们发现：1.25 mmol/L乙醛酸抑制生长，但0.25和0.5 mmol/L不会，允许比较较低浓度下的聚集体形成;乙醛酸诱导的多糖产生，其随着乙醛酸浓度和时间的增加而增加;随着乙醛酸添加量的增加，结合在一起的细胞的相对丰度增加;以及乙醛酸刺激的多糖水平的增加与聚集体大小直接相关。本研究表明乙醛酸可用于微藻生理生态学的研究，并为预测营养胁迫对多糖产量的影响提供了一种方法。
To determine how polysaccharide production influences microalgal aggregate size, we stimulated polysaccharide production using existing methods (previously applied to cyanobacteria and plants). Cultures were treated with glyoxylate (0, 0.25, 0.5, 1.25mmol/L) to simulate nutrient stress and examined for 9days (approaching stationary phase on day 9) to assess: growth rate, polysaccharide production (soluble, bound, and total), aggregate size, and the relation between polysaccharide and aggregate size. We found: 1.25mmol/L glyoxylate inhibits growth, but 0.25 and 0.5mmol/L do not, allowing comparisons of aggregate formation at lower concentrations; glyoxylate-induced polysaccharide production, which increased with increased glyoxylate concentration and time; an increase in relative abundance of cells bound together with increased glyoxylate addition; and increased glyoxylate-stimulated polysaccharide levels were directly correlated with aggregate size. This study indicates that glyoxylate may be used to examine microalgal ecophysiology and offers a method to predict the influence of nutrient stress on polysaccharide production.