Isolation of PCV3 from Perinatal and Reproductive Cases of PCV3-Associated Disease and In Vivo Characterization of PCV3 Replication in CD/CD Growing Pigs
Isolation of PCV3 from Perinatal and Reproductive Cases of PCV3-Associated Disease and In Vivo Characterization of PCV3 Replication in CD/CD Growing Pigs
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DOI：
10.3390/v12020219
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发表时间：
2020-02-01
期刊：
影响因子：
4.7
通讯作者：
Gimenez-Lirola, Luis
中科院分区：
文献类型：
--
作者：
Mora-Diaz, Juan;Pineyro, Pablo;Gimenez-Lirola, Luis
猪圆环病毒3型（Porcine Circovirus 3，PCV 3）是一种常见的猪源性病毒，可引起死胎、木乃伊胎、围产期脑炎和心肌炎以及生长期猪的动脉周围炎。从弱产仔猪或高产死胎和木乃伊胎中分离出3株PCV 3分离株。使用宏基因组测序确定来自不同传代和分离株（PCV 3a 1 ISU 27734、PCV 3a 2 ISU 58312、PCV 3c ISU 44806）的全长基因组序列。通过qPCR、IFA和原位杂交确认细胞培养物中的病毒产生。在实验条件下，在CD/CD猪（n = 8）中也证实了PCV 3的体内复制。病毒血症，首次检测到在7 dpi，检测到所有猪的28 dpi。在5/8头接种PCV 3的猪中，在7-14 dpi之间检测到IgM抗体应答，但在整个研究期间未检测到IgG血清转化。猪出现以心肌炎和系统性血管周围炎为特征的多系统炎症的组织学病变。通过原位杂交证实病毒在所有组织中复制。在临床上，所有动物在整个研究期间均无异常。尽管PCV 3的临床相关性仍存在争议，但这是首次从PCV 3相关疾病的围产期和生殖病例中分离PCV 3，并在CD/CD猪模型中对PCV 3感染进行体内表征。
Porcine circovirus 3 (PCV3) has been identified as a putative swine pathogen with a subset of infections resulting in stillborn and mummified fetuses, encephalitis and myocarditis in perinatal, and periarteritis in growing pigs. Three PCV3 isolates were isolated from weak-born piglets or elevated stillborn and mummified fetuses. Full-length genome sequences from different passages and isolates (PCV3a1 ISU27734, PCV3a2 ISU58312, PCV3c ISU44806) were determined using metagenomics sequencing. Virus production in cell culture was confirmed by qPCR, IFA, and in situ hybridization. In vivo replication of PCV3 was also demonstrated in CD/CD pigs (n = 8) under experimental conditions. Viremia, first detected at 7 dpi, was detected in all pigs by 28 dpi. IgM antibody response was detected between 7-14 dpi in 5/8 PCV3-inoculated pigs but no IgG seroconversion was detected throughout the study. Pigs presented histological lesion consistent with multi systemic inflammation characterized by myocarditis and systemic perivasculitis. Viral replication was confirmed in all tissues by in situ hybridization. Clinically, all animals were unremarkable throughout the study. Although the clinical relevance of PCV3 remains under debate, this is the first isolation of PCV3 from perinatal and reproductive cases of PCV3-associated disease and in vivo characterization of PCV3 infection in a CD/CD pig model.