Oocyte development and polypeptide dynamics during ovarian maturation in the red-claw crayfish Cherax quadricarinatus
Oocyte development and polypeptide dynamics during ovarian maturation in the red-claw crayfish Cherax quadricarinatus
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DOI：
10.1080/07924259.2000.9652402
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发表时间：
2000-01-01
影响因子：
0.8
通讯作者：
Sagi, A
中科院分区：
文献类型：
--
作者：
Abdu, U;Yehezkel, G;Sagi, A
红螯螯虾的卵巢发育分为两个主要阶段，即初级和次级卵黄发生。存在两种解剖学上不同类型的初级卵黄发生卵巢：一种包含均匀的乳白色白色卵母细胞（在染色质、染色质核仁、早期核周和晚期核周阶段），而另一种包含两种不同颜色的卵母细胞群体，最小的卵母细胞是均匀的乳白色白色，最大的是黄色至橙子（即，在脂质阶段）。次级卵黄发生卵巢的特征是存在一个同步生长的大卵母细胞群以及所有前四个初级卵黄发生阶段的卵母细胞。同步群由卵黄期经早熟期进入成熟期。相对低分子量（65-95 kDa）的多肽在含有染色质的初级卵黄发生卵巢至脂质期卵母细胞中丰富。在次级卵黄发生卵巢（主要由卵黄期卵母细胞组成）和新产的卵中检测到相对较高分子量（>100 kDa）的多肽。在次级卵黄发生过程中，血淋巴的高密度脂蛋白（HDL）部分含有四种次级卵黄发生特异性多肽（208，196，177和80 kDa），这些多肽不存在于初级卵黄发生雌性的血淋巴中。本研究阐明了卵巢和血淋巴中特异性多肽的出现顺序与性腺成熟、卵母细胞发育的关系。四脊肌
Ovarian development in the red claw crayfish Cherax quadricarinatus is divided into two major phases, namely primary- and secondary-vitellogenesis. Two anatomically distinctive types of primary-vitellogenic ovary are present: one contains uniform milky white oocytes (in the chromatin, chromatin-nucleolus, early-perinuclear and late-perinuclear stages), while the other contains two diversely colored oocyte populations, the smallest oocytes being uniform milky white and the largest being yellow to orange (i.e., at the lipid stage). Secondary-vitellogenic ovaries are characterized by the presence of a synchronously growing large oocyte group together with oocytes of all the first four, primary-vitellogenic stages. The synchronous group develops from the yolk stage via the prematuration stage into the maturation stage. Polypeptides of relatively low molecular masses (65-95 kDa) are abundant in the primary-vitellogenic ovary containing chromatin to lipid-stage oocytes. Polypeptides of relatively higher molecular masses (>100 kDa) were detected both in the secondary-vitellogenic ovary (composed mainly of yolk-stage oocytes) and in newly laid eggs. During secondary vitellogenesis, the high-density lipoprotein (HDL) fraction of the hemolymph contains four secondary vitellogenic-specific polypeptides (208, 196, 177 and 80 kDa) that are not present in the hemolymph of the primary vitellogenic female. In this study we have elucidated the relationship between the progress of gonad maturation, oocyte development and the sequence of appearance of specific polypeptides in the ovary and the hemolymph of C. quadricarinatus.