A NEW GENE (MADI) INVOLVED IN THE PHOTOTROPIC RESPONSE OF PHYCOMYCES
A NEW GENE (MADI) INVOLVED IN THE PHOTOTROPIC RESPONSE OF PHYCOMYCES
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DOI：
10.1007/bf00315807
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发表时间：
1990-08-01
期刊：
影响因子：
--
通讯作者：
ALVAREZ, MI
中科院分区：
文献类型：
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作者：
CAMPUZANO, V;DIAZMINGUEZ, JM;ALVAREZ, MI
已知只有八个基因参与藻毒素的向光反应（madA-H）。这些基因中受影响的突变体在该系统中的光敏转导过程的分析中发挥了重要作用。通过互补和重组研究了由Alvarez等（1989）分离的一组新的突变体，这些突变体不能向暗淡的单侧蓝光弯曲。这些突变体中的两个具有madE突变，一个具有madF突变，一个是双madE madF突变体。测试的其余三个突变体彼此不互补，并且与在基因madA、madB和madC中携带突变的菌株显示阳性互补，表明它们在命名为madI的新基因中携带突变。同源性分析表明，madI与madA、madB和madC不相关。
Only eight genes are known to be involved in the phototropic response of Phycomyces (madA-H). Mutants affected in these genes have played a major role in the analysis of photosensory transduction processes in this system. A set of new mutants isolated by Alvarez et al. (1989) that are unable to bend towards dim unilateral blue light were studied by complementation and recombination. Two of these mutants have mutations in madE, one has a mutation in madF and one is a double madE madF mutant. The three remaining mutants tested did not complement each other and showed positive complementation with strains carrying mutations in the genes madA, madB, and madC, indicating that they carried mutations in a new gene designated madI. Recombination analysis showed that madI is unlinked to madA, madB and madC.