Self-organization mechanism in a bone-like hydroxyapatite/collagen nanocomposite synthesized in vitro and its biological reaction in vivo
Self-organization mechanism in a bone-like hydroxyapatite/collagen nanocomposite synthesized in vitro and its biological reaction in vivo
复制标题
DOI：
10.1016/s0142-9612(00)00305-7
复制
发表时间：
2001-07-01
期刊：
影响因子：
14
通讯作者：
Tanaka, J
中科院分区：
文献类型：
--
作者：
Kikuchi, M;Itoh, S;Tanaka, J
当骨因损伤和/或疾病而丢失时，由于人工骨材料存在生物亲和性问题，通常用天然骨填充缺损。然而，天然骨也有供应和感染的问题。如果一种人造材料具有与骨相同的生物特性，它就可以代替天然骨进行移植。以Ca(OH)(2)、H3PO4和猪胶原为原料，采用同步滴定共沉淀法合成了羟基磷灰石（HAp）和胶原蛋白（Col）复合材料。在200 MPa的冷等静压作用下，复合材料的机械强度为骨的四分之一。它还显示了与移植物骨相同的生物学特性：材料被破骨细胞样细胞吞噬吸收，并引导成骨细胞在周围区域形成新骨。该HAp/Col复合材料具有相似的纳米结构和组成，可以替代自体骨移植物。2001爱思唯尔科学有限公司版权所有。
When bone is lost due to injury and/or illness, the defects are generally filled with natural bone because artificial bone materials have problems of bioaffinity. However, natural bone also has supply and infection problems. If an artificial material has the same biological properties as bone, it can replace natural bone for grafting. We synthesized a hydroxyapaite (HAp) and collagen (Col) composite by a simultaneous titration coprecipitation method using Ca(OH)(2), H3PO4 and porcine atelocollagen as starting materials. The composite obtained showed a self-organized nanostructure similar to bone assembled by the chemical interaction between HAp and Col. The consolidated composite by a cold isostatic pressure of 200 MPa indicated a quarter of the mechanical strength of bone. It also indicated the same biological properties as grafted bone: The material was resorbed by phagocytosis of osteoclast-like cells and conducted osteoblasts to form new bone in the surrounding area. This HAp/Col composite having similar nanostructure and composition can replace autologous bone grafts. (C) 2001 Elsevier Science Ltd. All rights reserved.