Identification of the Omega4499 regulatory region controlling developmental expression of a Myxococcus xanthus cytochrome P-450 system.
Identification of the Omega4499 regulatory region controlling developmental expression of a Myxococcus xanthus cytochrome P-450 system.
复制标题
鉴定控制黄色粘球菌细胞色素 P-450 系统发育表达的 Omega4499 调节区。
DOI：
10.1128/jb.181.17.5467-5475.1999
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发表时间：
1999
影响因子：
3.2
通讯作者：
Kroos,L
中科院分区：
文献类型：
--
作者：
Fisseha,M;Biran,D;Kroos,L
Ω4499是黄粘球菌染色体上的Tn5 lac插入位点，该位点将lacz表达融合到发育调节的启动子上。发育过程中发生的细胞间相互作用，包括C信号传导，是Tn5正常表达所必需的lacΩ4499。Ω4499插入的上游DNA已被克隆，启动子已被定位。对启动子下游DNA序列的分析显示，一个完整的开放阅读框和一个被Tn5 lacΩ4499中断的部分开放阅读框。这些开放阅读框的预测产物与细菌细胞色素P-450系统的还原酶和氧化酶成分高度相似，这些系统允许分解代谢或合成代谢不寻常的化合物。然而，Ω4499位点的基因产物的功能尚不清楚，因为em。含有Tn5 lacΩ4499的黄原菌在生长、发育聚集、子实体形成或孢子形成方面没有明显缺陷。对Ω4499调控区的缺失分析表明，在转录起始位点上游超过500 bp的多个DNA元件对发育启动子活性有贡献。至少有两个DNA元件，一个在- 49 bp下游，一个在- 49和- 218 bp之间，在响应细胞间C信号时提高了启动子的活性。Ω4499启动子区域的三个序列位于- 55、- 33和- 1 bp，与其他C信号依赖启动子中的一个7 bp序列几乎匹配。我们建议这些序列，与共识序列5 ‘ -CAYYCCY-3 ’相匹配，被称为C盒序列，我们推测这些序列是对m的表达重要的作用调控元件。黄原基因在发育过程中依赖于细胞间C信号传导。
Ω4499 is the site of a Tn5 lacinsertion in theMyxococcus xanthuschromosome that fuseslacZexpression to a developmentally regulated promoter. Cell-cell interactions that occur during development, including C signaling, are required for normal expression of Tn5 lacΩ4499. The DNA upstream of the Ω4499 insertion has been cloned, and the promoter has been localized. Analysis of the DNA sequence downstream of the promoter revealed one complete open reading frame and a second partial open reading frame that is interrupted by Tn5 lacΩ4499. The predicted products of these open reading frames are highly similar to reductase and oxidase components of bacterial cytochrome P-450 systems, which allow catabolism or anabolism of unusual compounds. However, the function of the gene products of the Ω4499 locus remains unclear becauseM. xanthuscontaining Tn5 lacΩ4499 exhibits no apparent defect in growth, developmental aggregation, fruiting body formation, or sporulation. Deletion analysis of the Ω4499 regulatory region showed that multiple DNA elements spanning more than 500 bp upstream of the transcriptional start site contribute to developmental promoter activity. At least two DNA elements, one downstream of −49 bp and one between −49 and −218 bp, boosted activity of the promoter in response to intercellular C signaling. Three sequences in the Ω4499 promoter region, centered at −55, −33, and −1 bp, nearly match a 7-bp sequence found in other C signal-dependent promoters. We propose that these sequences, matching the consensus sequence 5′-CAYYCCY-3′, be called C box sequences, and we speculate that these sequences arecis-acting regulatory elements important for the expression ofM. xanthusgenes that depend upon intercellular C signaling during development.